首页|Screening and functional analysis of the long-range interaction elements of β-globin genes

Screening and functional analysis of the long-range interaction elements of β-globin genes

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Objective: Studies have shown that β-globin gene presents a selective expression transformation mechanism during development, and its upstream locus control region (LCR) regulates the expression pattern of β-globin gene family. To further explore the molecular network of β-globin gene expression regulation, other long-range regulatory elements that may be involved in the regulation of β-globin gene expression were screened and the dynamic regulation and transformation mechanism of β-globin gene was deeply studied. Methods: Promyelocytic cells were induced to differentiate by all-trans retinoic acid. β-globin gene promoter region and LCR were used as the target sites for circular chromosome conformational capture (4C) analysis. Through sequencing and regulatory element analysis, the sites interacting with β-globin family loci were screened in the whole genome. Results: According to the results of 4C sequencing, the sites that interact with HBD promoter region and LCR were screened. Verified by chromosome conformational capture (3C), the results were consistent with those of sequencing. The functional analysis of regulatory elements by formaldehyde-assisted separation regulatory elements and Epiregio online website showed that the screening sites AC105129.4, AL354707.17, AC078785.22 and AC021646.35 were all potential regulatory elements involved in β-globin gene. Conclusion: The interaction between 4C screening site and anchor site showed the complex spatial organization of β-globin family loci in the nucleus.

β-Globin geneCircular chromosome conformation captureChromatin interactionChromosome conformation capture

XIAO Yi-shu、XU Lan、LIU Chun-ya、DU Le、SUN Yuan-tian、LI Dong-na、REN Li-cheng

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Department of Biology,Hainan Medical University,Haikou 571199,China

国家自然科学基金High-level Talents Project of Hainan Natural Science FoundationInnovation Project for Graduate Students in Hainan Province

31660318820RC638Hys2020-377

2022

海南医科大学学报(英文版)

海南医科大学学报(英文版)

ISSN：
年,卷(期)：2022.28(24)
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