首页|Expression and correlation of pyruvate kinase M2 and miRNA-122 in sepsis associated AKI

Expression and correlation of pyruvate kinase M2 and miRNA-122 in sepsis associated AKI

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Objective: To investigate the expression and correlation of pyruvate kinase M2 and miRNA-122 in sepsis associated acute kidney injury (S-AKI). Methods: A mouse model of S-AKI was induced by cecal ligation and perforation (CLP), and normal mice were used in the control group. Serum and renal tissue were collected from each group, respectively, and the levels of blood urea nitrogen (BUN) and creatinine (Cr) were detected by biochemical analyzer. The levels of lactate in serum and kidney tissue of mice in each group were detected by colorimetric method. Real-time quantitative PCR (RT-qPCR) was used to detect the expression of miRNA-122 and pkm2 mRNA in kidney tissue in each group. Western blotting was used to detect the expression of PKM2 protein in kidney tissue in each group. Pearson's method was used to analyze the pairwise correlation of miRNA-122, PKM2 and lactate levels. Results: (1) Compared with the control group, the levels of BUN and Cr increased significantly after 12 h and 24 h of CLP treatment (P <0.05). (2) Compared with the control group, after 12 h and 24 h of CLP treatment, the levels of lactate in the serum and kidney tissue of the mice were significantly higher than those in the control group (P<0.05). (3) Compared with the control group, the expression of miRNA-122 in renal tissue began to decrease at 4 h after CLP, and decreased significantly at 12-24 h (P<0.05). Compared with the control group, the expression of pKm2 mRNA and protein in renal tissue began to increase after 4 h of CLP, and increased significantly at 12-24 h (P<0.05). (4) Correlation analysis showed that miRNA-122 was significantly negatively correlated with lactate level (P<0.0001, r=-0.7167). There was a significant positive correlation between pkm2 mRNA and lactate level (P<0.0001, r=0.6817). There was a significant negative correlation between miRNA-122 and pkm2 mRNA expression (P<0.0001, r=-0.8122). Conclusion: In S-AKI, dysregulated expression of miRNA-122 may aggravate the occurrence and development of AKI by regulating the level of PKM2, and promoting aerobic glycolysis and lactate levels.

miRNA-122PKM2SepsisAcute kidney injuryAerobic glycolysis

FU Zhou、LI Ting、SONG Xu、LI Ru-xin、WANG Kai-li、WU Yu-tao、HU Nuo-ya、QUAN Kang-li、DU Bang-zhe

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Undergraduates of Changzhi Medical College,Changzhi 046000,China

Department of Physiology,Changzhi Medical College,Changzhi 046000,China

Innovation and Entrepreneurship Training Program for College Students in Shanxi ProvinceInnovation and Entrepreneurship Training Program for College Students in Shanxi ProvinceInnovation and Entrepreneurship Training Program for College Students in Shanxi Province国家自然科学基金山西省自然科学基金山西省高等学校科技创新项目Scientific Research Programs of Health Commission of Shanxi ProvinceProject of Academic Technology Leader in Changzhi Medical College

202208462020385201939981902020201801D2214442019L06622018126XSQ201903

2022

海南医科大学学报(英文版)

海南医科大学学报(英文版)

ISSN：
年,卷(期)：2022.28(24)
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