首页|Effect of Drynaria total flavonoids on the expression of NMDAR1, GluR2 and CaMK Ⅱ in the brain of hydrocortisone model mice

Effect of Drynaria total flavonoids on the expression of NMDAR1, GluR2 and CaMK Ⅱ in the brain of hydrocortisone model mice

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Objective: To study the effect of total flavonoids of Rhizoma Drynariae on the expression of NMDAR1, GluR2 and CAMKⅡ protein in model mice of kidney deficiency induced by exogenous glucocorticoid hydrocortisone and its mechanism.Methods: Kunming (KM) mice were randomly divided into the blank group, the hydrocortisone model group, the anti-brain failure capsule group, the Drynaria total flavonoids group, the Drynaria total flavonoids+ER blocker group, with 15 animals in each group. Except for the blank group, all groups were injected intramuscularly with hydrocortisone (25 mg·kg-1·d-1) to create models. The water maze experiment, new object recognition experiment and platform jump experiment were used to conduct behavioral investigations. HE staining was used to observe the pathological changes of mouse hippocampus, and Western blotting detected the expressions of NMDR1, GluR2 and CAMKⅡ proteins in the hippocampus of mice in each group. Results: The experimental results showed that compared with the model group, the learning and memory ability of the mice in the Drynaria fortunei total flavonoids group was significantly improved, and the difference was statistically significant (P <0.01), the expression of NMDR1 and GluR2 proteins in the hippocampus of the mice was significantly increased (P<0.01), and the level of CAMKⅡ protein significantly decreased (P<0.01). Conclusion: The total flavonoids of Rhizoma Drynariae may enhance the expression of NMDAR1 and GluR2 protein in the brain of hydrocortisone model mice through ER, reduce the expression of CAMKⅡ protein, and alleviate the damage to the brain tissue of the model mice and play a neuroprotective effect.

Total flavonoids of DrynariaeRhizomaAlzheimer's diseaseNMDAR1GluR2CaMK ⅡER

WANG Yi-tong、XU Yan-ming、ZHANG Zhi-bo、SUN Hui-feng、ZHANG Ning、YANG Bo

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School of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,China

College of Jiamusi,Heilongjiang University of Traditional Chinese Medicine,Jiamusi 154007,China

国家自然科学基金面上项目国家重点研发计划Scientific and Technological Achievements Research and Development and Cultivation Project of Heilongjiang Provincial UniversitiResearch Project of Heilongjiang Provincial Health CommissionHeilongjiang University of Traditional Chinese Medicine Research FundHeilongjiang University of Traditional Chinese Medicine Research FundHeilongjiang University of Traditional Chinese Medicine Research Fund

816735812017YFC1700701TSTAU-C20180202020-3032018jkcy052018pt042019BJP01

2022

海南医科大学学报(英文版)

海南医科大学学报(英文版)

ISSN：
年,卷(期)：2022.28(24)
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