首页|Investigation of paeonol-geniposide on acute alcoholic liver injury based on uniform design method
Investigation of paeonol-geniposide on acute alcoholic liver injury based on uniform design method
扫码查看
点击上方二维码区域，可以放大扫码查看
原文链接
NETL
NSTL
万方数据
维普
Objective: To explore the optimal ratio and compatibility effect of paeonol-geniposide combination on acute alcoholic liver injury by uniform design. Methods: Lieber-DeCarli alcoholic liquid feed was used to induce acute alcoholic liver injury in mice. Uniform design was used to select the best dosage combination of paeonol and geniposide, and the related indexes of liver injury and oxidative stress were detected by kit. Serum inflammatory factors were detected by ELISA, and the expressions of p38 MAPK, JNK and NF-κB P65 related proteins in liver were detected by Western-blot. Results: The regression equation suggested that paeonol: geniposide = 220: 20 was the best ratio of paeonol and geniposide to resist alcoholic liver injury. Compared with the model group, the liver injury indexes and oxidation products of the paeonol+geniposide group decreased significantly, the antioxidant activity of liver tissue increased significantly, and the expression levels of p-p38 MAPK, p-JNK and NF-κB P65 protein decreased significantly. Conclusion: The optimal dosage of paeonol-geniposide was effectively optimized by uniform design and pharmacodynamic analysis. The combination of the two drugs could reduce the alcoholic liver injury by reducing the oxidative stress injury and inflammatory response in the liver tissue of mice, and its effect might be related to the targeting of p38 MAPK/JNK/NF-κB channel.
PaeonolGeniposideAlcoholic liver injuryUniform designChinese medicine effective ingredient ratio
LIU Ke、LIU Yu-long、SUN Min、LIU Ling-ling、ZHANG Lu-ning、YAN Gui-ming
展开 >
Anhui University of Chinese Medicine,Hefei 230012,China
NETL
NSTL
万方数据
维普
