首页|Abnormal expression of TGFβ1 in acute myeloid leukemia and its regulation effect on leukemia cells
Abnormal expression of TGFβ1 in acute myeloid leukemia and its regulation effect on leukemia cells
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NETL
NSTL
万方数据
维普
Objective: To explore the level of acute myeloid leukemia (COX-2), transforming growth factor β1 (TGFβ1), basic fibroblast growth factor (bFGF), and vascular endothelial growth factor (VEGF) in acute myeloid leukemia (AML) patients and to investigate the role of TGFβ1 on the proliferation, apoptosis and cycle of AML cells, providing new targets and research bases for the treatment and prognostic evaluation of AML. Methods: Peripheral blood was collected from 80 AML patients and 60 normal people. The levels of COX-2, TGFβ1, bFGF and VEGF in peripheral blood mononuclear cells and serum were determined by using quantitative polymerase chain reaction and enzyme linked immunosorbent assay, respectively. The proliferation, apoptosis and cycle of AML cells affected by overexpression and silencing of TGFβ1 was detected using cell counting kit-8 and flow cytometry. Results: The expression of COX-2, TGFβ1, bFGF and VEGF increased significantly in peripheral blood mononuclear cells and serum in AML patients. TGFβ1 promoted AML cell proliferation, inhibited its apoptosis, and increased stage G2 cell proportion. Conclusion: COX-2, TGFβ1, bFGF and VEGF play important roles in the progression of AML. TGFβ1 is a new potential biomarker for the diagnosis and treatment of AML.
Acute myeloid leukemiaDiagnostic targetsProliferation of apoptosisTGFβ1
NETL
NSTL
万方数据
维普
