Molecular Mechanism Study on the Resistance of Multidrug-resistant Pseudomonas to Carbapenems and Aminoglycosides Antibiotics
[Objective]To explore the molecular mechanism of resistance of multidrug-resistant Pseudomonas to carbapenems and aminoglycosides antibiotics.[Methods]Six strains of multidrug-resistant Pseudomonas aerugi-nosa and Pseudomonas aeruginosa were received from different wards of our hospital from January 2022 to Decem-ber in the laboratory department.The bacterial species were identified and verified using the Vitek-2 compact fully automated identification instrument and 16s rDNA sequence primers.The E-test drug sensitivity test was used to determine the antibacterial concentration of the strain against clinical antibiotics.The gene subtypes of aminoglyco-side and carbapenemase methylases were identified through PCR amplification and sequence comparison.[Results]Among the 6 strains of Pseudomonas genus bacteria,4 strains were Pseudomonas aeruginosa and 2 strains were Pseudomonas putida,both of which were Carba NP positive and produced carbapenemase;The strains showed re-sistance to penicillin,carbapenems,aminoglycosides,cephalosporins,and tetracycline antibiotics.Among the 6 strains,only SY456 was resistant to aflatoxin with a MIC value of 92μg/mL,while the other strains were media-tors or sensitive to aflatoxin resistance.The Carba NP method screened 6 strains of bacteria,all of which produced drug-resistant B-class carbapenemases.The overlap PCR sequence comparison showed that six strains carried ar-mA gene and blaIMP-45 gene,and three strains of Pseudomonas putida SY47,SY153,and SY434 had significant-ly different pulse field gel electrophoresis(PFGE)bands.[Conclusion]There are both Pseudomonas aeruginosa and Pseudomonas aeruginosa carrying both armA and blaIMP-45 in our hospital.Clinical physicians need to adjust their medication strategies in a timely manner based on the type of drug-resistant strains.
万方数据
维普
