Expression Characteristics and Clinical Significance of LAG-3 in CD8+ T Cells from Patients with Chronic Hepatitis B Virus Infection
[Objective]To investigate the expression characteristics and clinical significance of LAG-3 in CD8+T cells from patients with chronic hepatitis B virus(HBV)infection.[Methods]A total of 60 patients with chronic HBV admitted to our hospital from April 2019 to April 2020 were selected as the HBV group.While 60 health examinees in our hospital during the same period were selected as the control group.Blood samples were collected from both groups.The expression level,intensity and frequency of LAG-3 in CD8+T cells were measured by flow cytometry.The expression levels of inter-leukin-10(IL-10),human leukocyte antigen-DR(HLA-DR),interferon-γ(IFN-γ)and transcription factor T-bet(T-bet)were measured.The mechanism of LAG-3 in regulating CD8+T cells was analyzed.[Results]The expression level and in-tensity of LAG-3 in the HBV group were higher than those in the control group(P<0.05).The expression of IL-10 in the LAG-3 positive subgroup was higher than that in LAG-3 negative subgroup within HBV group,and the expression of IL-10 in the LAG-3 negative and positive subgroups within HBV group was higher than that in the control group(P<0.05).The expression of HLA-DR in LAG-3 positive subgroup was lower than that in LAG-3 negative subgroup within HBV group,and the expression of HLA-DR in the LAG-3 negative and positive subgroups within HBV group was lower than that in the control group(P<0.05).The expression of IFN-gamma in the LAG-3 positive subgroup within HBV group was lower than that in the LAG-3 negative subgroup,and the expression of IFN-gamma in the LAG-3 negative and positive subgroups within HBV group was lower than that in the control group(P<0.05).The expression of T-bet in the LAG-3 positive subgroup of HBV group was lower than that in the LAG-3 negative subgroup,and the expression of IFN-gamma in negative and positive subgroups of HBV group was lower than that in the control group(P<0.05).[Conclusion]Chronic HBV infection results in the increase of LAG-3 in CD8+T cells under the actionof inflammation.LAG-3 can reduce the se-cretion of IFN-gamma by affecting the expression of IL-10,HLA-DR,IFN-gamma and T-bet,and increase the consump-tion of T cells.
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