[Objective]To explore the correlation of the bacterial biofilm phenotype and genotype with multidrug re-sistance in Escherichia coli.[Methods]A total of 230 clinical isolates of Escherichia coli were collected.Genotyping was performed using multiplex PCR,biofilm formation ability was determined using a modified crystal violet assay,and anti-biotic susceptibility was tested using the Kirby-Bauer method.Statistical analysis was conducted on the antibiotic resistance patterns of Escherichia coli,the relationship between bacterial biofilm phenotype and multidrug resistance,and the rela-tionship between Escherichia coli genotype and multidrug resistance.[Results]Escherichia coli showed high resistance rates to ampicillin,cefuroxime,and ciprofloxacin,but lower resistance to piperacillin and no resistance to imipenem.Bio-film phenotypes of Escherichia coli were classified as strong,moderate,weak and none,accounting for 3.91％,31.30％,57.83％and 6.96％,respectively.The common biofilm phenotypes in Escherichia coli were moderate and weak pheno-types.The stronger biofilm formation ability was associated with higher rate of multidrug resistance.The CTX-M-9 geno-type was the most prevalent,accounting for 35.65％.Spearman correlation analysis showed that the biofilm formation a-bility of Escherichia coli was positively correlated with multidrug resistance rate(P=0.012).[Conclusion]The biofilm formation ability of Escherichia coli is associated with multidrug resistance.Different genotypes exhibit resistance to multi-ple antibiotics,highlighting the need for strict regulation of antibiotic use in clinical settings.
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