Effects of SGLT-2 Inhibitor on Vascular Endothelial Cells in Diabetes Mice
[Objective]To investigate the effect of sodium glucose cotransporter 2(SGLT-2)inhibitor on vascular en-dothelial cells in diabetes mice.[Methods]Twenty four SPF 8-week old male ICR mice were intraperitoneally injected with low-dose streptozotocin(STZ)(50 mg/kg)to establish a diabetes model.The model was successfully established.They were randomly divided into a model group and a treatment group(Daggligin intervention treatment for 12 weeks).Six mice were used as the control group,and physiological saline was injected subcutaneously.Three groups were all fed with regu-lar feed,and the changes in body weight and blood glucose of mice were dynamically observed every week.The plasma levels of tumor necrosis factor alpha(TNF-α),interleukin-1β(IL-1β),monocyte chemotactic factor-1(MCP-1),superox-ide dismutase(SOD),and malondialdehyde(MDA)were compared among the three groups of mice.[Result s]From the 2nd weekend to the 12th weekend,both the model group and the treatment group had lower body weight than the control group,while the treatment group had higher body weight than the model group,with a statistically significant difference(P＜0.05).Compared with the control group,the blood glucose levels in the model group and treatment group were sig-nificantly increased,and the difference was statistically significant(P＜0.05);Compared with the model group,the blood glucose levels of the treatment group mice significantly decreased from the 3rd to the 12th weekend,and the differ-ence was statistically significant(P＜0.05).The plasma levels of TNF-α,IL-1β,and MCP-1 in the model group were significantly higher than those in the control group,while the plasma levels of TNF-α,IL-β,and MCP-1 in the treatment group were significantly lower than those in the model group,with statistical significance(P＜0.05).The plasma SOD level in the model group was lower than that in the control group,while the plasma SOD level in the treatment group was higher than that in the model group,with a statistically significant difference(P＜0.05);Compared with the control group,the plasma MAD level in the model group was higher than that in the control group,while the plasma MAD level in the treatment group was lower than that in the model group,with a statistically significant difference(P＜0.05).[Conclusion]SGLT-2 inhibitor can effectively inhibit the release of inflammatory factors from vascular endothelial cells in diabetes mice,and improve the function of vascular endothelial cells.
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