Relationship of Th1/Th2 Cell Status in Patients with Pulmonary Tuberculosis with Microbial Communities and Expression of Notch2 mRNA and IL-27 mRNA in BALF
[Objective]To investigate the correlation of the Th1/Th2 cell status in patients with pulmonary tuberculosis(TB)with the microbial communities and expression of Notch2 and interleukin-27(IL-27)in bron-choalveolar lavage fluid(BALF).[Methods]A total of 776 TB patients were divided into an active group(n=370)and a non-active group(n=406).Additionally,352 patients with non-TB respiratory diseases were se-lected as the control group.The microbial levels in the BALF,the mRNA expression levels of Notch2 mRNA and IL-27 mRNA from BALF,and the peripheral blood Th1/Th2 cell ratio were measured to analyze the cor-relations of Th1/Th2 with microbial communities and Notch2 and IL-27 mRNA expression in BALF.[Re-sults]Both active and non-active groups had lower cell number in peripheral blood Th1 and Th2 cells,the ratio of Th1/Th2 was also lower if compared to the control group.The active group had a lower proportion of Th1 cellsand Th1/Th2 ratio than the non-active group(P＜0.05),but there were no statistically significant differences in Th2 cell proportions between the two groups(P＞0.05).Both active and non-active groups had higher counts of Haemophilus,Neisseria and higher IL-27 mRNA expression compared to the control group,while Streptococcus counts and Notch2 mRNA expression were lower(P＜0.05).Moreover,the active group had higher counts of Haemophilus and Neisseria and higher IL-27 mRNA expression,but lower Streptococcus counts and lower Notch2 mRNA expression than the non-active group(P＜0.05).Pearson correlation analysis showed that the Th1/Th2 ratio in TB patients was positively correlated with Streptococcus counts and Notch2 mRNA expression(P＜0.05),and negatively correlated with Haemophilus and Neisseria counts and IL-27 mRNA expression(P＜0.05).[Conclusion]The Th1/Th2 cell ratio is low in TB patients and further decrea-ses with disease progression.This immune imbalance is associated with infections by Haemophilus,Neisseria,and Streptococcus in the BALF and with Notch2 mRNA and IL-27 mRNA expression.
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