Expression of Ang-1 and MCP-1 in Peripheral Blood of Children with Bronchial Asthma and Their Correlation with Lung Function
Objective To investigate the expression of angiopoietin-1(Ang-1)and chemoattractant protein-1(MCP-1)in the peripheral blood of bronchial asthma children and their correlation with lung function.Methods A total of 162 children with bronchial asthma diagnosed and treated in Kaifeng Children's Hospital from May 2019 to May 2022 were selected as the research object and set as the research group.According to the Global Initiative for Asthma,they were divided into mild persistent group(92 cases),moderate persistence group(37 cases)and severe persistence group(33 cases).At the same time,80 healthy subjects were included as the control group.The enzyme-linked immunosorbent was used to measure MCP-1 and Ang-1 levels,and pulmonary function meters were used to measure the lung function[forced expiratory volume in one second/forced vital capacity(FEV1/FVC),forced expiratory volume in one second(FEV1),peak expiratory flow rate(PEF)]of children in each group.The correlation between Ang-1 and MCP-1 peripheral blood levels and lung function were investigated by Pearson analysis.Results Compared with the control group,the expression level of Ang-1 in the peripheral blood of children with asthma was lower,and the level of MCP-1 was higher(P＜0.05).Peripheral blood Ang-1 levels of the moderate and severe children were lower than those of the mild children(severe＜moderate＜mild),and MCP-1 levels were higher than those of the mild children(severe＞moderate＞mild)(P＜0.05).Compared with the control group,the detection indicators of FEV1/FVC,FEV/,PEF and other indicators in children with asthma were lower(P＜0.05).The indicators of FEV1/FVC,FEV1 and PEF in children with moderate and severe asthma were lower than those in mild patients(severe＜moderate＜mild)(P＜0.05).Peripheral blood Ang-1 in children with asthma was positively correlated with lung function indexes such as FEV1/FVC,FEV1,PEF(r=0.753,0.784,0.605,P＜0.05).MCP-1 was negatively correlated with FEV1/FVC,FEV1,PEF and other lung function indexes(r=-0.724,-0.815,-0.618,P＜0.05).The area under the curve and 95％CI of Ang-1 and MCP-1 for the diagnosis of childhood bronchial asthma were 0.801(0.746-0.856)and 0.803(0.778-0.828),the diagnosed cut-off values 17.88 μg·L-1 and 58.47 ng·L-1,respectively.The sensitivity and specificity of Ang-1 in diagnosing bronchial asthma in children were 85.54％and 76.25％.MCP-1 was effective in diagnosing bronchial asthma in children.The sensitivity and specificity were 83.84％and 77.50％.The combined detection of Ang-1 and MCP-1 can increase the sensitivity to 89.50％(P＜0.05).Conclusion The expression of Ang-1 in the peripheral blood of bronchial asthma children is low,and the expression of MCP-1 is high.The Ang-1 is positively correlated with lung function,and MCP-1 is negatively correlated.Ang-1 and MCP-1 have certain diagnostic value for children with bronchial asthma.
angiopoietin-1chemotactic protein-1childrenbronchial asthmalung function
万方数据
维普
