Effects of Conjugated Linoleic Acid on Gut Microbiota of Obese Mice with High Fat Diet
Objective To investigate the effects of conjugated linoleic acid(CLA)on gut microbiota of obese mice with high fat diet.Methods Twenty-four LDLR-/-mouse were randomly divided into 3 groups,with 8 per in each group:normal group mouse received normal diet and normal saline gavage,high fat group mouse received high fat diet and normal saline gavage,and intervention group mouse received high fat diet and CLA.All mice were intervened for 16 weeks,the body weight of the mice was recorded every 2 weeks,and oral glucose tolerance test(OGTT)was conducted at the 15th week.Mouse eyeball blood was extracted for detection of lipid and insulin indicators in serum.The relative expression of inflammatory factors and intestinal tight junction proteins zone occludens-1(ZO-1)and Occludin(Ocln)at the mRNA level were analyzed by RT-PCR.The characteristics of mice gut microbiota changes were analyzed by 16S rDNA gene sequencing.Results Compared with the normal group,the mice fed with high fat diet showed significant weight gain,glucose and lipid metabolism disorders,increased ileum inflammation and gut microbiota disorders.After CLA intervention,the mice in the intervention group lost weight(P=0.025),the glucose and lipid metabolism disorder improved,the relative expression of ileum inflammatory factors Toll-like receptor 4(TLR4),tumor necrosis factor-α(TNF-α)decreased significantly(P＜0.001)and intestinal tight junction protein ZO-1(P=0.006),Ocln(P=0.004)increased significantly,the sequencing results showed that the abundance of Firmicutes and Bifidobacterium increased.Conclusion CLA may increase the abundance of Firmicutes and Bifidobacterium by optimizing the gut microbiota structure of obese mice,thereby reducing the weight of obese mice,regulating the metabolism of glucose and lipids,reducing intestinal inflammation and repairing intestinal mucosal barrier.
万方数据
维普
