Expression of DHCR7 Gene in Gastric Cancer and Its Relationship with Immune-Related Genes
Objective To explore the expression of 7-dehydrocholesterol reductase(DHCR7)gene in gastric cancer and its relationship with immune-related genes.Methods UALCAN and TIMER databases were used for analyzing the expression of DHCR7 gene in different types of tumors.GEPIA,TCGA and GEO databases were used for investigating the expression of DHCR7 gene in gastric cancer.Kaplan-Meier Plotter database was used for evaluating the relationship between DHCR7 gene expression and the prognosis of gastric cancer patients.cBioPortal database was used for identifying the co-expressed genes with DHCR7 in gastric cancer,and the genes with higher correlation coefficients among the co-expressed genes were displayed.Geneontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)pathway analysis were performed to analyze the co-expressed gene with DHCR7.The correlation between DHCR7 gene and CD8+T cells,chemokines related to recruitment of CD8+T cells,as well as immune activation related-genes were evaluated.The relationship between the expression of DHCR7 and clinical pathological feature of gastric cancer patients were classified.Results DHCR7 gene had a high expression trend in most tumor types.The expression of DHCR7 gene in gastric cancer was higher than that in normal gastric mucosa.The results of 201790-s-at chip in Kaplan-Meier Plotter database showed that the survival of patients with high expression of DHCR7 was shorter.The GO and KEGG pathway enrichment analysis of negatively correlated genes with DHCR7 were mainly enriched in immune-related functions and signaling pathways.DHCR7 had a negative correlation with CD8+T cells,chemokines associated with recruiting CD8+T cells,and immune activation related-genes.The high expression of DHCR7 was positively correlated with the age of gastric cancer patients.Conclusion DHCR7 is highly expressed in gastric cancer tissue,and patients with high expression of DHCR7 have poor prognosis.The DHCR7 gene can affect the immune microenvironment of gastric cancer and is expected to become a new target for immunotherapy of gastric cancer.
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