Potential Gene Analyze for Distinguishing Lung Adenocarcinoma from Lung Squamous Cell Carcinoma
Objective To analyze the potential genes of non-small cell lung cancer(NSCLC),and to identify relevant genes that distinguish lung adenocarcinoma(LUAD)from lung squamous cell carcinoma(LUSC),and explore more effective methods for the diagnosis and treatment of NSCLC.Methods The differentially expressed genes(DEGs)in LUAD and LUSC tissue samples was analyzed by three Gene Expression Omnibus(GEO)databases(GSE4882,GSE7339 and GSE40275).Gene Ontology(GO)enrichment analysis was performed on DEGs and protein-protein interaction(PPI)network was constructed to identify key hub genes.Finally,the differential expression of key hub genes was verified in clinical samples.Results Twenty-two DEGs of LUAD and LUSC were found in GSE4882,GSE7339 and GSE40275 datasets.Six key hub genes(KRT18,RAN,NME1,NME2,MIF and CFB)were discovered by constructing a PPI network.The relationship between hub gene expression and survival in The Cancer Genome Atlas(TCGA)database was analyzed,and revealed that KRT18 may be a potential gene for distinguishing LUAD from LUSC.Meanwhile,a risk prediction model for KRT18 gene mutation and prognosis of LUAD and LUSC patients was constructed.Finally,clinical tissue samples from the First Affiliated Hospital of Xinxiang Medical College were used to verify the KRT18 expression.Conclusion KRT18 may be a potential gene marker to distinguish LUAD from LUSC.
万方数据
维普
