Correlation Analysis of p53 and TPX2 Expression Levels in Cervical Cancer Tissue and Radiotherapy Sensitivity
Objective To investigate the expression levels of tumor protein 53(p53)and Xklp2 target protein(TPX2)in cervical cancer tissue,and their correlation with radiotherapy sensitivity.Methods A total of 135 patients with cervical cancer who underwent radical radiotherapy in Anyang Cancer Hospital from January 2020 to June 2023 were selected.After 4-6 weeks of treatment,the patients were divided into radiotherapy sensitive group and radiotherapy resistant group based on the treatment effect evaluated with the RECIST criteria.The expression levels of p53 and TPX2 in cervical cancer tissue were detected by immunohistochemical method and RT-PCR.The relationship between clinical features such as p53 and TPX2 expression,age,Federation International of Gynecology and Obstetr(FIGO)staging,tissue differentiation,lymph node metastasis and radiation sensitivity in cervical cancer tissue was evaluated.Multivariate logistic regression analysis was used to predict factors related to radiation sensitivity in cervical cancer tissue,and the correlation between p53 and TPX2 expression in cervical cancer tissue was evaluated.Results All patients enrolled completed radiotherapy successfully.There were 64 cases of complete remission,40 cases of partial remission,25 cases of stable disease,and 6 cases of progressive disease.The sensitivity rate of radiotherapy was 77.04％(104/135).The relative expression levels and positive rates of p53 and TPX2 in cervical cancer tissues of the radiotherapy sensitive group were lower than those in the radiotherapy resistant group(P＜0.05).Compared with the radiation resistant group,the radiation sensitive group had a higher proportion of tissue differentiation with low to medium differentiation,no lymph node metastasis,and tumor diameter＜4 cm(P＜0.05).Logistic regression analysis showed that the expression of p53 and TPX2,as well as the degree of tissue differentiation were predictive factors for the radiosensitivity of cervical cancer tissue(P＜0.05).Correlation analysis showed that,there was a positive correlation between the relative expression levels of p53 and TPX2 in cervical squamous cell carcinoma tissue(r=0.229,P＜0.05).Conclusion p53 and TPX2 are highly expressed in cervical cancer tissue,and are associated with radiation sensitivity,which can provide reference for predicting the radiation sensitivity and prognosis of cervical cancer patients.
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