Evaluation of Diagnostic Performance of Plasma cfDNA Methylation Combined Detection in Diagnosis of Hepatocellular Carcinoma
Objective To investigate the clinical value of methylation of GNB4,TCF24,Riplet,ACP1,and TSPYL5 genes in plasma circulating free DNA(cfDNA)for the diagnosis of liver cancer.Methods Sliding window technology was used to screen differential methylation markers between liver cancer and normal tissues in public databases and analyze their methylation levels.Liver cancer,liver cirrhosis tissue,and healthy human leukocyte samples were collected from the First Affiliated Hospital of Zhengzhou University,and Sanger sequencing was used to detect the methylation levels of selected biomarkers,biomarkers with better sensitivity and specificity were selected.Plasma from 24 cases of liver cancer,23 cases of liver cirrhosis,and 99 healthy individuals were collected,and the diagnostic performance of the above markers alone and in combination for liver cancer were detected through methylation specific PCR.Results Five biomarkers(GNB4,TCF24,Riplet,ACP1 and TSPYL5)were significantly hypermethylated in liver cancer samples.The tissue sequencing results showed that except for TCF24,the methylation negative rate of other biomarkers in liver cirrhosis tissue was greater than 80.0％.In plasma sample validation,GNB4 alone had the highest AUC for diagnosing liver cancer,at 0.765,with a sensitivity of 66.7％and a specificity of 91.8％.In the multi gene combination,GNB4+TSPYL5 had the best diagnostic performance,with an area under curve(AUC)value of 0.893,sensitivity of 83.3％,and specificity of 90.2％.Conclusion Methylation of GNB4,Riplet,ACP1 and TSPYL5 can be used for liver cancer diagnosis,and the combined diagnostic performance is better than that of a single gene.
万方数据
维普
