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血站核酸检测实验室环境消毒监测结果与分析

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目的 通过监控及监测本站核酸检测实验室环境消毒结果,确保血液核酸检测结果的准确性与稳定性.方法 收集2021年6月至2023年6月本站核酸检测实验室3个工作区域25次监测结果,评估核酸检测实验室空气、设备、地面、物体表面的消毒方法对消毒效果的影响.结果 采集的25次环境监测结果中2021年10月标本处理、标本制备区移动车台面乙型肝炎病毒核酸(HBVDNA)阳性,复检后仍呈阳性,消毒后重新取样,结果为阴性,其余24次环境监测合格率均为100%.Pearson相关性分析结果显示,环境监测合格率与核酸检测结果的拆分阳性率无相关性(P>0.05).结论 本站核酸检测实验室环境消毒措施能有效确保检测质量.同时,监测结果中偶发的HBV DNA阳性结果系设备污染,未对检测实验室造成不良影响,但日常工作中应高度重视并加强实验室的清洁消毒及评估,确保实验室检测结果的准确和环境、人员等安全.
Environmental Disinfection and Monitoring of Blood Station Nucleic Acid Testing Laboratory
Objective To explore environmental disinfection and monitoring of Blood Station nucleic acid testing laboratory,analysis and evaluation of the efficacy.Methods From June 2021 to June 2023,the monitoring data of 25 sampling point of 3 working area of nucleic acid testing laboratory were collected,the disinfection effect of air,equipment,ground and surface of objects of nucleic acid testing laboratory were evaluated.Results Among the 25 environmental monitoring results collected,the specimen treatment in October 2021 and the mobile vehicle table in the specimen preparation area nucleic acid(HBV DNA)were positive,but still positive after retesting,ressampled after disinfection,and the results were negative.The qualified rate of the remaining 24 environmental monitoring was 100%.Pearson correlation analysis results showed that there was no correlation between the qualified rate of environmental monitoring and the split positive rate of the nucleic acid test results(P>0.05).Conclusion The disinfection measured of nucleic acid testing laboratory environment on this site can effectively ensure the quality of testing.The reactiveness of HBV DNA in environmental disinfection result was pollution of equipment,and there was no harmful effects,reconstruction and strengthen the disinfection of laboratory and evaluation working,can ensure the result accuratissime and security of environment.

nucleic acid testinglaboratoryenvironmental disinfectionenvironmental monitoring

李鹏

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开封市中心血站,河南开封 475000

核酸检测 实验室 环境消毒 环境监测

2024

河南医学研究
河南省医学科学院

河南医学研究

影响因子:0.979
ISSN:1004-437X
年,卷(期):2024.33(11)