Preliminary Exploration of the Effect of High Sugar on the Growth and Osteogenic Differentiation Ability of Stem Cells from Human Exfoliated Deciduous Teeth
Objective To observe the effect of high glucose on growth and osteogenic differentiation of stem cells from human exfoliated deciduous teeth(SHED)in vitro.Methods Retained deciduous teeth(healthy children's ages ranging from 6 to 8,without dental and periodontal disease)were obtained in the oral and maxillofacial surgery of SATH Hospital of Stomatology Henan University.SHED were gained by cultivated in vitro.The cultured SHED were divided into 5.5 mmol·L-1 low glucose concentration group and 25 mmol·L-1 high glucose concentration group.The cellular viability of the two groups were detected with CCK-8,respectively.After osteoinduction,the expression of calcium deposition by alizarin red staining on day 21,osteogenic differentiation related genes,such as alkaline phosphatase(ALP),Runt-related transcription factor 2(RUNX-2),osteocalcin(OCN)and osteopontin(OPN)by real time fluorogenic quantitative PCR(QPCR)in the two groups were observed respectively.Results The growth of the high glucose group was lower than the physiological group on day 2-6(P＜0.05).After osteoinduction,the number of calcified nodules in the high glucose group was less than that in the low glucose concentration group,and the expression of ALP,OPN,RUNX-2,OCN genes in high glucose group were lower than those in physiological group(P＜0.05).Conclusion High glucose can inhibit SHED's growth and osteogenic differentiation.
high glucosestem cells from human exfoliated deciduous teethin vitro culturecell growthosteoinductionosteogenic differentiation
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维普
