Objective To investigate the role of apoptosis and autophagy on hepatic injuryunder brain death state.Methods The 40 SD rats were randomly divided into 4 groups:the brain death group was induced by slow intermittent intracranial compression and maintained in a state of brain death for 6 hours,perform intracranial catheterization on the sham surgery group,but did not induce brain death,inject autophagy inhibitor 3-methyladenine(3-MA)intraperitoneally into the brain death+autophagy inhibitor group 1 hour before inducing brain death,and establish and maintain a state of brain death for 6 hours,for the brain death+blank solvent group intraperitoneal injection of blank solvent dimethyl sulfoxide(DMSO)was administered 1 hour before inducing brain death,and the brain death state was established and maintained for 6 hours.After the molding was established,the venous blood and liver specimens were taken from rats.qPCR was used to detect the expression of Caspase-3 mRNA.Western blot was used to detect the expression of LC3 and Caspase-3 protein.Immunohistochemistry was used to detect the distribution and expression of Caspase-3 in liver tissues.Results Compared with sham surgery group,the expression of apoptosis-related gene Caspase-3 was up-regulated and the apoptosis of hepatocytes increased in brain death group,the expressions of autophagy-related genes LC3 were up-regulated(P＜0.05).Compared with the DMSO group,there was no statistical difference in apoptosis-related genes and autophagy-related genes in brain death group(P＞0.05).Compared with the DMSO group,the expression of LC3 in 3-MA group was down-regulated(P＜0.05),the expression of apoptosis-related gene Caspase-3 was up-regulated(P＜0.05),and the apoptosis of hepatocytes increased(P＜0.05).Conclusion Apoptosis participates in and mediates the hepatocellular injury under brain death,and autophagy attenuates hepatocellular injury underbrain death state.
NETL
NSTL
万方数据
