Inhibiting Autophagy Increases Hepatocellular Injury Under Brain Death State
Objective To investigate the role of apoptosis and autophagy on hepatic injuryunder brain death state.Methods The 40 SD rats were randomly divided into 4 groups:the brain death group was induced by slow intermittent intracranial compression and maintained in a state of brain death for 6 hours,perform intracranial catheterization on the sham surgery group,but did not induce brain death,inject autophagy inhibitor 3-methyladenine(3-MA)intraperitoneally into the brain death+autophagy inhibitor group 1 hour before inducing brain death,and establish and maintain a state of brain death for 6 hours,for the brain death+blank solvent group intraperitoneal injection of blank solvent dimethyl sulfoxide(DMSO)was administered 1 hour before inducing brain death,and the brain death state was established and maintained for 6 hours.After the molding was established,the venous blood and liver specimens were taken from rats.qPCR was used to detect the expression of Caspase-3 mRNA.Western blot was used to detect the expression of LC3 and Caspase-3 protein.Immunohistochemistry was used to detect the distribution and expression of Caspase-3 in liver tissues.Results Compared with sham surgery group,the expression of apoptosis-related gene Caspase-3 was up-regulated and the apoptosis of hepatocytes increased in brain death group,the expressions of autophagy-related genes LC3 were up-regulated(P＜0.05).Compared with the DMSO group,there was no statistical difference in apoptosis-related genes and autophagy-related genes in brain death group(P＞0.05).Compared with the DMSO group,the expression of LC3 in 3-MA group was down-regulated(P＜0.05),the expression of apoptosis-related gene Caspase-3 was up-regulated(P＜0.05),and the apoptosis of hepatocytes increased(P＜0.05).Conclusion Apoptosis participates in and mediates the hepatocellular injury under brain death,and autophagy attenuates hepatocellular injury underbrain death state.
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