EB Virus Infection in Patients with Gastric Cancer and Its Effects on the Expressions of p53 and Bcl-2 in Cancer Tissues
Objective To explore the human Epstein-Barr(EB)virus infection in patients with gastric cancer and its effects on the expressions of p53 and B-cell lymphoma-2(Bel-2)in cancer tissues.Methods A total of 76 patients with gastric cancer admitted to Xinxiang Central Hospital from August 2019 to January 2023 were selected.In situ hybridization was used to detect the expressions of EB virus-encoded low molecule RNA in cancer tissues and adjacent tissues,and reverse transcription polymerase chain reaction(RT-PCR)was applied to detect the mRNA expressions of p53 and Bcl-2 in cancer tissues and adjacent tissues.The relationship of EB virus infection with clinicopathological features of gastric cancer and expressions of p53 and Bcl-2 in cancer tissues was analyzed.Results The positive rate of EB virus in cancer tissue samples of 76 patients with gastric cancer was 27.63％(21/76)and was 4.29％(3/76)in adjacent tissue samples.The positive rate of EB virus in cancer tissues of patients with gastric cancer was higher than that in adjacent tissues(P＜0.05).Compared with patients with EB virus-negative gastric cancer,EB virus-positive patients had high proportions of lesions in proximal stomach,tissue immersion to the serous layer and lymph node metastasis(P＜0.05).The mRNA expressions of p53 and Bcl-2 in cancer tissues were higher than those in adjacent tissues(P＜0.05).The mRNA expressions of p53 and Bcl-2 in cancer tissues of patients with EB virus infection were higher than those of patients without EB virus infection(P＜0.05).Conclusion EB virus infection is associated with proximal gastric lesions,tissue immersion to the serous layer and lymph node metastasis in patients with gastric cancer.EB virus can up-regulate p53 expression by driving host p53 gene methylation,and promote cancer cell growth in collaboration with Bcl-2,which may provide clinical evaluation factors for gastric cancer diagnosis and treatment and new targets for immunotherapy.
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