Expression of PI3K/AKT Signaling Pathway Related Proteins in Hepatocellular Carcinoma and Their Predictive Value for Survival After Hepatectomy
Objective To investigate the expression of phosphatidylinositol-3 kinase(PI3K)/protein kinase B(AKT)signaling pathway related proteins in hepatocellular carcinoma and its predictive value for survival after hepatectomy.Methods A total of 143 patients with hepatocellular carcinoma admitted to Puyang Oilfield General Hospital from March 2017 to March 2020 were selected as the study subjects.All patients underwent hepatectomy.The subjects were followed up and divided into survival group and death group according to the follow-up results.The relative expression levels of PI3K/AKT signaling pathway related proteins[PI3 K,phosphorylated protein kinase B(p-AKT),mammalian target of rapamycin(p-mTOR)]were detected by Western blot,and the relative expression levels of PI3K,p-AKT,and p-mTOR were compared between hepatocellular carcinoma and paracancer tissues.Receiver operating characteristic(ROC)curve was used to analyze the optimal cut-off values of PI3 K,p-AKT and p-mTOR in predicting postoperative death of hepatocellular carcinoma,and the optimal cut-off values were grouped.Kaplan-Meier method was used to draw the survival curve and analyze the survival of patients.Cox regression analysis was used to analyze the risk factors affecting postoperative survival of hepatocellular carcinoma.Results The levels of PI3K,p-AKT and p-MTOR in hepatocellular carcinoma were higher than those in paracancer tissues(P＜0.05).The survival rates of patients in 1,2 and 3 years were 86.67％,74.81％and 52.59％.The levels of microvascular invasion,tumor number(multiple),lymph node metastasis,age,alpha fetoprotein(AFP),PI3 K,p-AKT,p-mTOR and tumor diameter in death group were higher than those in survival group(P＜0.05).ROC curve showed that the area under the curve of PI3 K,p-AKT and p-mTOR in predicting postoperative death of hepatocellular carcinoma were 0.736,0.782 and 0.813,respectively.The survival curve showed that compared with the high PI3K group,the survival rate of HCC patients in the low PI3K group was higher(P＜0.05).Compared with the high p-AKT group,the survival rate of HCC patients in the low p-AKT group was higher(P＜0.05).Compared with the high p-mTOR group,the survival rate of hepatocellular carcinoma patients in the low p-mTOR group was higher(P＜0.05).Cox regression analysis showed that age,abnormal levels of AFP,PI3 K,p-AKT,p-mTOR and microvascular invasion were risk factors for postoperative death of hepatocellular carcinoma(P＜0.05).Conclusion The abnormal expression of PI3 K,p-AKT and p-mTOR in hepatocellular carcinoma tissues is a risk factor for postoperative death in patients with hepatocellular carcinoma,and the three factors have high predictive value for postoperative death in patients with hepatocellular carcinoma.
万方数据
维普
