Objective To investigate the in vitro efficacy of electrolyzed silver ions in inactivating human immunodeficiency virus(HIV).Methods HIV-1 was used to infect C8166 cells and rapidly replicate within them,with subsequent release into the cell culture supernatant,leading to the formation of syncytia in C8166 cells.When the cell density reached 1 × 107 cells·mL-1,electrolyzed silver ions were mixed with the C8166 cells and incubated at room temperature for 1 hour.The resulting mixture was then used as the test sample.Under an inverted microscope,the cytopathic effect induced by HIV-1 Ⅲ B in each well was observed,and syncytia within the wells were detected.The Spearman-Karber method was used to calculate the TCID50 values of the virus in the samples.Results The residual virus titer in the control group was 106 67 TCID50·mL-1,while that in the sample group was 102 5 TCID50·mL-1.Compared with the virus control group,the sample group showed a reduction in HIV-1 virus titer of ≥4.17 log units under room temperature conditions for 1 hour,corresponding to an inactivation rate of 99.993％.Conclusion In vitro experiments indicate that electrolyzed silver ions can effectively inactivate HIV,suggesting potential for use in the prevention and treatment of AIDS.
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