Experimental Study on Inactivation of HIV by Electrolytic Silver Ion
Objective To investigate the in vitro efficacy of electrolyzed silver ions in inactivating human immunodeficiency virus(HIV).Methods HIV-1 was used to infect C8166 cells and rapidly replicate within them,with subsequent release into the cell culture supernatant,leading to the formation of syncytia in C8166 cells.When the cell density reached 1 × 107 cells·mL-1,electrolyzed silver ions were mixed with the C8166 cells and incubated at room temperature for 1 hour.The resulting mixture was then used as the test sample.Under an inverted microscope,the cytopathic effect induced by HIV-1 Ⅲ B in each well was observed,and syncytia within the wells were detected.The Spearman-Karber method was used to calculate the TCID50 values of the virus in the samples.Results The residual virus titer in the control group was 106 67 TCID50·mL-1,while that in the sample group was 102 5 TCID50·mL-1.Compared with the virus control group,the sample group showed a reduction in HIV-1 virus titer of ≥4.17 log units under room temperature conditions for 1 hour,corresponding to an inactivation rate of 99.993％.Conclusion In vitro experiments indicate that electrolyzed silver ions can effectively inactivate HIV,suggesting potential for use in the prevention and treatment of AIDS.
万方数据
维普
