Study on the Mechanism of miR-556-3p Inhibiting Its Expression Through Targeted Binding to DAB2IP in Promoting Breast Cancer Progression
Objective To investigate the expression of miR-556-3p in breast cancer and elucidate the mechanism by which miR-556-3p promotes the progression of breast cancer by targeting DAB2IP.Methods Retrospective analysis was made on 65 breast cancer tissue samples from January 2018 to May 2019 in the Second Affiliated Hospital of Zhengzhou University,and 30 normal breast tissues were included as the control group.Real-time PCR was used to detect the expression of miR-556-3p in breast cancer tissues,control groups and breast cancer cell lines.The patients were followed up for 5 years,and the survival rate of the patients was counted.In vitro experiments,MTT test,transwell test,luciferase reporter gene and cell transfection were used to detect the effect of miR-556-3p on the activity and invasion of breast cancer cells.Western blot and Real-time PCR methods were used to detect the effects of miR-556-3p on the DAB2IP and MAPK pathways.The targeting relationship between miR-556-3p and DAB2IP was analyzed using a luciferase reporter system.Results miR-556-3p expression levels in breast cancer patient tissues were higher than in control group samples.The overall survival rate of breast cancer patients with high miR-556-3p expression was lower than that of the low expression patients.In vitro experiments showed that changes in miR-556-3p expression in breast cancer cells were positively correlated with cell viability and invasion.In the MCF-10A and MCF-7 cell lines,overexpression of miR-556-3p increased cell viability and invasion,while knockdown of miR-556-3p decreased these parameters.The expression of DAB2IP was directly regulated by miR-556-3p,and after overexpression of miR-556-3p,the protein levels of DAB2IP,Erk,and p-Erk in breast cancer cells decreased,while knockdown of miR-556-3p led to an increase in these protein levels.Conclusion miR-556-3p targets the 3'-UTR region of DAB2IP,thereby blocking DAB2IP mRNA translation and promoting the proliferation of MCF-10A and MCF-7 cells.miR-556-3p plays a key role in the occurrence and development of breast cancer,providing a new theoretical basis for targeted prevention and treatment of breast cancer.
breast cancermiR-556-3pDAB2IPcell proliferationMCF-10A cell lineMAPK pathway
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维普
