Application Value of Metagenomic Second-Generation Sequencing in Etiological Diagnosis of Bone and Joint Infection
Objective To analyze the application value of metagenomic second-generation sequencing(mNGS)in the etiological diagnosis of bone and joint infection.Methods A total of 63 patients with suspected bone and joint infection admitted to Zhengzhou Orthopaedic Hospital from April 2020 to April 2023 were selected as the study objects.Traditional culture method and mNGS detection were performed.Clinical comprehensive diagnosis was taked as the"gold standard".The detection results and sensitivity of bone and joint infection detected by traditional culture method and mNGS were analyzed.The results of identification of pathogenic bacteria types were compared between traditional culture method and mNGS detection method.The changes of white blood cell count(WBC),erythrocyte sedimentation rate(ESR)and C-reactive protein(CRP)in patients with osteojoint infection were compared before and after treatment.Results Of 63 suspected patients with bone and joint infection,41 were positive and 22 were negative.The traditional culture method produced 25 true positive cases and 19 true negative cases.There were 34 true positive cases and 20 true negative cases detected by mNGS.The sensitivity of mNGS was 82.93％(34/41),higher than that of traditional culture method[60.98％(25/41)](P＜0.05).Forty-three strains of pathogenic bacteria were cultured by traditional culture method,and 51 strains were detected by mNGS.Staphylococcus aureus and coagulase negative staphylococcus were the main pathogens,followed by enterobacter cloacae,enterococcus,brucella,streptococcus and Mycobacterium tuberculosis.The levels of WBC,ESR and CRP after targeted therapy based on mNGS results were lower than those of empirical therapy(P＜0.05).Conclusion The application of mNGS detection in the etiological diagnosis of bone and joint infection can improve the diagnostic sensitivity,accurately identify the types of infection pathogens,and improve the efficacy of anti-infection treatment.
bone and joint infectionmetagenomic second-generation sequencingetiology
万方数据
维普
