Effects of different concentrations of TGF-β1 on the number and transdifferentiation of epithelial cells in endometrial tissues
Objective To explore different concentrations of transforming growth factors-β1(transforming growth factor-β1.TGF-β1)the effect of on the number and transdifferentiation of endometrial epithelial cells(EECs)in en-dometrial tissue.Methods Totally 40 cases of normal endometrial tissue obtained from surgery in our hospital were col-lected,and epithelial cells were isolated.According to the random number table method,the cells were divided into 10 normal control groups(with only culture medium added)and 10 experimental groups(with culture medium and different concentrations of TGF)-β1)30 copies,of which the experimental group is further divided into three groups,namely low-dose TGF-β1 set of 10,medium dose TGF-β1 set of 10,high-dose TGF-β1 group of 10,with high,medi-um,and low dose groups receiving TGF-β1(9 ng/mL,6 ng/mL,3 ng/mL).Measure the number of living cells,the content of type Ⅰ collagen(Col Ⅰ)and fibronectin(FN)in cell suspension,and the content of cell cytoplasm α-Smooth muscle actin(α-SMA and E-cadherin).Results That the secretion of FN in the normal control group was(0.469±0.010)ng/L,and the secretion of Col Ⅰ was(0.532±0.033)ng/L;The difference between the FN values measured in each experimental group and the Col-Ⅰ values was statistically significant(P＜0.01);Compared with the normal control group,TGF-β1 to E-cadherin and α-SMA expression has the opposite effect,and TGF-β1,differ-ent concentrations have different effects,specifically manifested as TGF-β1 As the concentration gradually increases,the amount of E-cadherin in the cytoplasm will gradually decrease.On the contrary,The amount of α-SMA in the cytoplasm will gradually increase(P＜0.05).The difference was statistically significant(F=145.125,179.511,P＜0.01).Conclusion Different concentrations of TGF-β1 can stimulate an increase in the number of epithelial cells in endometrial tissue,induce their transformation into myofibroblasts,and TGF-β1 the higher the concentration,the stronger the effect.
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