Clinical value,biological function and potential mechanism of LINC00978 in head and neck squamous cell carcinoma
Objective To investigate the prognostic value of long non-coding RNA(LncRNA)LINC00978 in head and neck squamous cell carcinoma(HNSCC)and its effects on the growth and apoptosis of FaDu cells,and to analyzed its po-tential mechanism.Methods Cancer databases GEPIA and UALCAN were used to analyze the expression level of LINC00978 in HNSCC samples and normal samples,and the relationship between the LINC00978 expression and clinical parameters of patients.The relative expression levels of LINC00978 in human HNSCC cell lines(FaDu,SCC4)and one oral mucosa cell line(HOK)were detected by quantitative real-time fluorescence PCR(qRT-PCR).Functional en-richment analysis of LINC00978 was performed based on the Kyoto Encyclopedia of Genes and Genomes(KEGG).The growth and apoptosis of FaDu cells were detected by CCK-8 assay and flow cytometry,respectively.Direct interaction be-tween LINC00978 and METTL3 protein was confirmed by RNA immunoprecipitation(RIP).Western blot was used to de-tect the changes of METTL3 protein in FaDu cells after LINC00978 silence.Results Compared with normal tissue sam-ples,the expression level of LINC00978 in HNSCC tissue samples was significantly increased(P＜0.0001).Compared with HOK cells,the expression levels of LINC00978 in FaDu and SCC4 were significantly up-regulated(P＜0.05).High expression of LINC00978 was significantly correlated with tumor stage,grade,age,gender,overall survival and dis-ease-free survival in patients with HNSCC.KEGG enrichment analysis indicated that LINC00978 may be involved in me-tabolism,DNA replication,apoptosis and other biological pathways in HNSCC.Compared with negative control siRNA(si-NC),silencing of LINC00978(si-LINC00978#1 and si-LINC00978#2)could significantly inhibit the growth of FaDu cells(P＜0.05)and promote cell apoptosis(P＜0.05).LINC00978 and METTL3 protein have direct interaction in FaDu cells.Silencing of LINC00978 resulted in decreased METTL3 protein levels in FaDu cells.Over-expression of METTL3 can obviously save the inhibitory effect of LINC00978 knockdown on the proliferation of FaDu cells.Conclusion The expression of LINC00978 as a cancer promoting molecule is up-regulated in HNSCC,and is a prognos-tic indicator of poor survival.LINC00978 may maintain the survival of HNSCC by binding and regulating METTL3.
LINC00978Head and neck squamous cell carcinomaPrognosisCell proliferationMETTL3
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