摘要
目的 探讨右美托咪定(DEX)对小鼠肾脏缺血再灌注(I/R)肾小管和微循环损伤的机制和影响.方法 将雄性C57BL/6小鼠分为对照组、I/R组、DEX组和I/R-DEX组各10只;对照组不进行任何处理;I/R组用血管钳将左肾动静脉夹闭40 min后再灌注3 h;DEX组输注10 μg/kg盐酸右美托咪定;I/R-DEX组在缺血开始时输注10 μg/kg盐酸右美托咪定后同I/R组处理.比较4组氧化状态参数、肾功能参数、肾脏氧合变量和组织学表现.结果 I/R组超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽(GSH),以及肾皮质(CμPO2)、外髓质(MμPO2)和肾静脉(PrvO2)微血管氧张力低于对照组和DEX组,I/R-DEX组上述氧化状态参数和肾脏氧合变量低于对照组和DEX组,但高于I/R组.I/R组丙二醛(MDA),血尿素氮(BUN)、血清肌酐(Scr)、肿瘤坏死因子α(TNF-α)和胱抑素C(Cys C),以及组织学损伤评分高于对照组和DEX组,I/R-DEX组上述肾功能参数和组织学损伤评分高于对照组和DEX组,但低于I/R组.镜下I/R组可观察到广泛的间质出血,皮质损伤,肾小球细胞减少和萎缩,皮质小管出现上皮细胞脱落,I/R-DEX组肾小球形态正常,小管和间质区显示轻微变性.结论 缺血开始时输注10 μg/kg DEX可改善小鼠氧化状态、微循环状态和I/R相关的肾损伤,对肾脏缺血再灌注具有保护作用.
Abstract
Objective To investigate the mechanism and effect of Dexmedetomidine(DEX)on renal tubule and microcir-culation injury during renal ischemia reperfusion(I/R)in mice.Methods Male C57BL/6 mice were divided into control group,I/R group,DEX group and I/R-DEX group with 10 mice each.The control group did not receive any treatment;In I/R group,the left renal arteriovenous was clamped for 40 min and then reperfusion was performed for 3 h.DEX group was given 10µg/kg Dexmedetomidine hydrochloride.The I/R-DEX group was treated with the I/R group after infusion of 10μg/kg Dexmedetomidine hydrochloride at the onset of ischemia.The oxidation status parameters,renal function pa-rameters,renal oxygenation variables and histological findings of the 4 groups were compared.Results SOD,CAT and GSH,as well as CμPO2,MμPO2 and PrvO2 in I/R group were lower than those of control group and DEX group,the a-bove oxidation state parameters and kidney oxygenation variables in I/R-DEX group were lower than those of control group and DEX group,but higher than those of I/R group.MDA,BUN,Scr,TNF-α,Cys C and histological injury score in I/R group were higher than those of control group and DEX group,and those of I/R-DEX group were higher than those of control group and DEX group,but lower than those of I/R group.Microscopically,I/R group showed exten-sive interstitial bleeding,cortical injury,glomerular cell reduction and epithelial cell shedding in atrophic cortical tubules,while I/R-DEX group showed normal glomerular morphology and slight degeneration in tubule and interstitial are-a.Conclusion Infusion of 10μg/kg DEX at the beginning of ischemia can improve the oxidative status,microcirculation status and I/R-related renal injury in mice,and has a protective effect on renal ischemia-reperfusion.
基金项目
河南省重点研发与推广专项(222102310019)
河南省医学科技攻关省部重点项目(SBGJ202102209)
郑州市中心医院科研启动基金项目(KYQDJJ2021005)
NETL
NSTL
万方数据