Expression of Lim domain kinase 1 in renal clear cell carcinoma and effect on proliferation and migration of tumour cells
Objective The purpose of this study was to investigate the expression of LIM domain kinase 1(LIMK1)in clear cell renal cell carcinoma(ccRCC)and its effect on tumor cell proliferation and migration.Methods The data was obtained in February 2023.Transcriptome data and clinical information of ccRCC patients were obtained from The Cancer Genome Atlas(TCGA)database.The impact of LIMK1 on the prognosis of ccRCC patients was evaluated through sur-vival analysis,univariate,and multivariate analysis.Using functional enrichment analysis to predict the molecular signa-ling pathways through which LIMK1 is involved in the pathogenesis of ccRCC.The expression level of LIMK1 in ccRCC cell lines was detected using RT-qPCR technology,and LIMK1 expression in 786-O cell lines was reduced using gene interference technology.Comprehensive assessments of the effects of LIMK1 on cell proliferation and migration,were conducted through CCK8 experiments,and scratch healing experiments.Results Results from the public database showed that LIMK1 was highly expressed in ccRCC tissues(P＜0.05),and patients with high expression had a poorer prognosis(P＜0.05).Univariate and multivariate regression analysis showed that LIMK1 was an independent prognostic risk factor for ccRCC patients.Functional enrichment analysis revealed that elevated LIMK1 expression might be in-volved in the pathological process of ccRCC through the T-cell receptor signaling,P53 signaling,Toll-like receptor sig-naling,and JAK/STAT signaling pathways.The experimental results indicate that the expression level of LIMK1 is in-creased in ccRCC cell lines,Knockdown of LIMK1 expression could significantly inhibit the proliferation and migration ability of 786-O cells(P＜0.05).Conclusion LIMK1 is a potential diagnostic marker for poor prognosis and may in-fluence the onset and progression of ccRCC by modulating properties such as tumour cell proliferation and invasion.
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