Effect of microRNA-let-7a in invasion and apoptosis in middle ear cholesteatoma keratinocyts
Objective This study aims to investigate the effects of microRNA-7a in molecular mechanisms that control apoptosis and invasion in ear cholesteatoma keratinocyts.Methods Totally 65 cholesteatoma tissues received through op-eration between Auguest 2017 and March 2021 in the First Affiliated Hospital of Zhengzhou University,and the tissues were processed for cell culture.The keratinocytes of cholesteatoma in middle ear were transfected with lentiviral vectors that mediate microRNA-let-7a mimics,and they were divided into the microRNA-let-7a mimics group,the Mock group,and NC group.Real time-PCR was used to assess the expression levels of microRNA-let-7a.The growth trend of the cells was observed in the three group.FCM,AnnexinV-FITC and Transwell were used to assess the cell cycle,in-vasion and apoptosis of cholesteatoma keratinocytes,respectively.Results The cell growth of microRNA-let-7a mim-ics group was lower than that of other two group(P＜0.05).The cell cycle distribution of the microRNA-let-7a mimics group,Mock group and NC group showed significant differences,with a marked increase in the proportion of choleateatoma cells in the G0/G1 phase,which was statistically significant.The early apoptosis rate of cholesteatoma keratinocytes in Mock group and NV group was significantly lower than that in microRNA-let-7a mimics group(P＜0.05).The cell number of cholesteatoma keratinocyte invasion chamber in microRNA-let-7a mimics group was significantly higher than that in the other two groups(P＜0.05).Conclusion The present study revealed that microRNA-let-7a promotes the apoptosis and invasion,inhibits proliferation and growth of cholesteatoma keratinocytes.
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