Evaluation of utilization value of fiber-like substance after crushing Spatholobus Suberectus based on HPLC content determination
Objective To establish the HPLC method with gradient elution for the content determination of catechin and epicatechin in fine powder and fiber-like substance after crushing Spatholobus Suberectus,so as to provide reference for e-valuation of utilization value of fiber-like substance.Methods The gradient elution was performed on a RD-C18 col-umn(4.6 mm ×250 mm,5 μm)with 0.1％phosphoric acid aqueous solution as the mobile phase A and acetonitrile as the mobile phase B.The flow rate was 0.8 mL/min and the wavelength was 260 nm.The column temperature was 30 ℃.The content difference of catechin and epicatechin in fine powder and fiber-like substance was analyzed by t-test with SPSS 26.0 software.Results The linear ranges of catechin and epicatechin were 0.045 4-0.227 0 mg/mL(R2=0.999 7)and 0.062 2-0.311 0 mg/mL(R2=0.999 3),respectively.The average recoveries were 102.31％and 98.70％,respec-tively.The results of t-test showed that there was no significant difference between the contents of catechin and epicate-chin in fine powder and fiber-like substance after crushing Spatholobus Suberectus(P＞0.05).Conclusion The estab-lished method is simple,sensitive and accurate,which can provide a reference for evaluating the utilization value of fiber-like substance after crushing Spatholobus Suberectus.The fine powder and the fiber-like substance have certain quality equiv-alence in main active components,so it is not recommended to discard fiber-like substances.
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