Analysis of onserological characteristics of HBsAg-HBV DNA+voluntary blood donors in Zhengzhou
Objective To understand the characteristics of hepatitis B serological markers in voluntary blood donors with HBsAg non-reactive HBV DNA reactivity(HBsAg-HBV DNA+)in Zhengzhou.Methods After simultaneously tested by two different ELISA reagents for HBsAg,anti-HCV and anti-HIV1/2,562 666 blood donors samples(ex-cept for ELISA dual-reactive samples)was performed by Nucleic Acid Testing(NAT).HBsAg-HBV DNA+sam-ples were collected for supplementary hepatitis B serological test by chemiluminescence detection.Results Totally 351 cases were HBV DNA reactive,and the residual risk rate of HBV was reduced to 624/10 000 after NAT.351 HBsAg-HBV DNA+samples were tested by chemiluminescence detection,of which 5 samples were HBsAg positive;346 sam-ples were HBsAg-HBV DNA+,of which 30 samples(8.67％)were window period infection,and 316(91.33％)samples were occult HBV infection;the proportion of the hepatitis B serological HBcAb positive pattern was 33.33％,the proportion of HBsAb and HBcAb positive was 20.51％,the proportion of HBeAb and HBcAb positive was 17.09％;the proportion of HBsAb Quantitative value＜10 IU/L was 60.40％,the proportion of HBsAb quantification value＞100 IU/L was 15.10％.The re-detection coincidence rate of HBV DNA in the HBsAb-positive group was 37.41％,which was significantly lower than that in the HBsAb-negative group(59.43％,x2=16.292,P＜0.001).Among the 351 HBsAg-HBV DNA+blood donors,the positive rate of HBcAb increased with the increase of age,and the highest positive rate(92.80％)was in the age group of 46-55.Conclusion HBV infection was mostly occult HBV infection,presenting a specific serological pattern.HBcAb-posi-tive and HBsAb-negative blood donors in the＞26-year-old age group may have a higher risk of transfusion-trans-mitted HBV.The application of NAT can effectively reduce the residual risk of HBV transmission through blood transfu-sion and improve the safety of blood.
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