Protective effects of carvacrol pretreatment on blood-brain barrier in rats with cerebral ischemia/reperfusion
Objective To investigate the protective effects of carvacrol pretreatment on cerebral ischemia/reperfusion in-jury(CI/RI)in rats and its effects on blood-brain barrier.Methods SD rats were divided randomly into sham,mod-el,low dose of carvacrol(50 mg/kg),high dose of carvacrol(100 mg/kg)and positive control(edaravone 10 mg/kg)groups.Drugs were administered by intraperitoneal injection for 1 week before the model operation.Middle cerebral ar-tery occlusion(MCAO)model was established by the line-bolus method,and the rats were ischemic for 60 min and then reperfused for 24 h.After the reperfusion,the neurological function of MCAO rats was measured by the Zea Longa method,blood-brain barrier permeability was evaluated by evans blue(EB)leakage method,the expression of zonula occludens-1(ZO-1)and occludin in the cortical semidarkness zone were detected by Western blot method,and the the relative gene expression of MMP-2 and MMP-9 in the cortical semidarkness zone were examined by RT-PCR.Results MCAO rats in the model group all had a higher degree of neurological dysfunction.Compared with the model group,high and low doses of carvacrol significantly reduced the neurological function scores of MCAO rats(P＜0.05),evidently reduced the volume of cerebral infarction(P＜0.05),significantly reduced the EB leakage from the ischemic side of the brain tissue(P＜0.05),significantly increased the expression of the blood-brain barrier tight junction proteins,ZO-1 and occludin(P＜0.05),and sig-nificantly reduced the relative gene expression of MMP-2 and MMP-9 in the cortical semidarkness zone(P＜0.01).Conclusion Carvacrylol pretreatment maintains the structural integrity of the blood-brain barrier and attenuates CI/RI by inhibiting the expression of MMP-2 and MMP-9.
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