Effect and molecular mechanisms of miR-182-5p on proliferation and migration of cervical cancer cells
Objective To investigate the effect of miR-182-5p on the biological characteristics of cervical cancer cells and its mechanism.Methods Cervical cancer and paracancer tissue samples collected in Zhengzhou People's Hospital from January 2019 to January 2022 were selected,and the expression of miR-182-5p in the above tissues was ana-lyzed by fluorescence quantitative PCR.Lentiviruses carrying empty vectors and miR-182-5p were infected with cer-vical cancer Hela cells,respectively,and miRNA control(miR-control group)and miR-182-5p overexpression cell lines(miR-182-5p group)were constructed.5-ethynyl-2'-deoxyuridine(EdU)staining and cell counting kit-8(CCK8)were used to analyze the proliferation ability of two group cells,and scratch and Transwell were used to analyze the migration ability of two group cells.The target gene of miR-182-5p was detected by bioinformatics and dual luciferase reporter gene,and the expression of the target gene was verified by Western blot.Results The expression level of miR-182-5p in cervical cancer tissues(0.31±0.02)was significantly lower than that in paracancer tissues(1.01±0.11,t=11.105,P＜0.05).The expression level of miR-182-5p in miR-182-5p group(2.77±0.43)was significantly higher than that in miR-control group(1.11±0.09,t=11.963,P＜0.05).The cell proliferation ability of miR-182-5p group(1.23±0.05)was signifi-cantly lower than that of miR-control group(1.93±0.07,t=14.771,P＜0.05).The positive rate of EdU staining in miR-182-5p group(20.33±1.63)％was significantly lower than that in miR-control group(40.32±1.86)％,and the difference was statistically significant(t=13.997,P＜0.05).The number of migrated cells in miR-182-5p group(56.18±4.22)was significantly lower than that in miR-control group[(157.63±8.25),t=18.958,P＜0.05].The cell scratch healing rate of miR-182-5p group(21.39±3.09)％was significantly lower than that of miR-control group[(76.17±4.22)％,t=18.145,P＜0.05].Rab27A was identified as the target gene of miR-182-5p by bioinformatics and dual luciferase genes.The Rab27A protein expression level in miR-182-5p group(2.18±0.19)was significantly higher than that in miR-control group(1.14±0.11,t=8.327,P＜0.05).Conclusion miR-183-5p inhibits the proliferation and migration of cervical cancer cells by upregulating Rab27.
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