Effect of pseudomonas aeruginosa mannose-sensitive hemagglutinin on skin repair in rats with deep second-degree burn
Objective To investigate the repair effect of pseudomonas aeruginosa mannose-sensitive hemagglutinin(PA-MSHA)on the skin of rats with deep second-degree burns,and to observe the healing rate and histopathological changes of bum wounds through the application of different doses of PA-MSHA,in order to provide new treatment ideas for bum treat-ment.Methods The rats with deep second-degree bum model were constructed by temperature control scalder.The rats were randomly divided into normal saline group,moist bum cream group,PA-MSHA 8 × 108/mL group,20 × 108/mL group,50 × 108/mL group and 125 × 108/mL group,with 10 rats in each group.The rats in each administration group were given the corresponding drug,twice a day in the morning and afternoon,and continued to be given until the wound healed.The healing of the burn wound and the histopathologic changes of the burned skin were observed in each group.Results Compared with the normal saline group,the bum area of PA-MSHA 125 × 108/mL group was reduced on day 7 and day 14,and the difference was statistically significant(P＜0.05).Compared with normal saline group,the burn area in moist burn cream group was increased,and the difference was statistically significance(P＜0.05).At the later stage of bum treatment,there was no significant difference in burn area between normal saline and PA-MSHA treatment groups(P＞0.05).Compared with the normal saline group,the skin tissue of moist burn cream group and PA-MSHA group showed thicker new epidermal layer,and the connective tissue and new blood vessels in the dermis increased significantly.Conclusion PA-MSHA can repair the skin of deep second-degree burn model rats,and its mechanism may be related to reducing the inflammation of burn wound and promoting angiogenesis.
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