Role of RNA m6A methyltransferase METTL3 in Ang Ⅱ-induced HK-2 cell injury
Objective To investigate the role of methyltransferase-like factor 3(METTL3)in hypertensive renal injury by using angiotensin Ⅱ(Ang Ⅱ)induced human kideny-2(HK-2)cell injury model and explore the related molecular mechanism.Methods HK-2 cells were stimulated by 5*10-4M Ang Ⅱ for 48 h to construct the hypertensive renal in-jury cell model,which was divided into control group,5*10-4M Ang Ⅱ group,siMETTL3 group and siMETTL3+AngⅡ group.To study the effect of Ang Ⅱ stimulation on METTL3 mRNA and protein expression and cell proliferation and ap-optosis in HK-2 cells.Results Ang Ⅱ could significantly increase the expression of METTL3 protein and mRNA in HK-2 cells compared with control group(P＜0.01).After METTL3 knockdown,the overall levels of METTL3 protein and mRNA in siMETTL3+Ang Ⅱ group were lower than those in Ang Ⅱ group(P＜0.05),and HK-2 cells in siMETTL3+Ang Ⅱ group were less proliferating and more apoptotic than those in Ang Ⅱ group.Conclusion METTL3 may have a protective effect on renal tubular epithelial cells from injury.METTL3 may be damaged after administration,and the protective increase of METTL3 may be induced,while the protective protein METTL3 may be knocked down at the same time after administration,leading to further cell damage.
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