Correlation analysis of peripheral blood lymphocyte subsets and TNF-α and IL-6 and IFN-γ with children with mycoplasma pneumoniae infection
Objective To investigate the correlation between peripheral blood lymphocyte subpopulations,tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and γ-interferon(IFN-γ)and children with Mycoplasma pneumoni-ae infection.Methods The clinical data of 86 children with Mycoplasma pneumoniae infection admitted to the Zhengzhou University Affiliated Luoyang Central Hospital from August 2022 to November 2023 were selected for retrospective analysis,and 43 healthy children who underwent physical examination in the Zhengzhou University Affiliated Luoyang Central Hospi-tal during the same period were selected as a healthy control group in the ratio of 2:1,and the levels of peripheral blood lymphocyte subpopulations(CD4+,CD8+,CD4+/CD8+),TNF-α,IL-6,and IFN-γ were compared between the two groups.Differences in peripheral blood lymphocyte subpopulations,TNF-α,IL-6,and IFN-γ levels were ana-lyzed by ROC curves and the value of the combined data in diagnosing Mycoplasma pneumoniae infection.Results Com-pared with the healthy control group,the Mycoplasma pneumoniae infection group had lower CD4+,CD4+/CD8+,and higher CD8+,TNF-α,IL-6,and IFN-γ(P＜0.05).Binary logistic regression analysis showed that CD4+,CD8+,CD4+/CD8+,TNF-α,IL-6,and IFN-γ were influential factors in children with Mycoplasma pneumoniae infections(P＜0.05).Receiver operating characteristic(ROC)curve analysis showed that peripheral blood lymphocyte subsets(CD4+,CD8+,CD4+/CD8+),TNF-α,IL-6,IFN-γ levels to diagnose Mycoplasma pneumoniae infection had area under the curve(AUCs)of 0.834,0.852,0.919,0.751,0.762,and 0.756,respectively.Peripheral blood lymphocyte subsets(CD4+,CD8+,CD4+/CD8+),TNF-α,IL-6,and IFN-γ levels were included in a logistic regression mod-el,and the regression coefficients were used to The joint value was derived,and the AUC of the joint data for diagnosing Mycoplasma pneumoniae infection was 0.945,with a sensitivity of 90.7％and a specificity of 97.7％.Conclusion Children with Mycoplasma pneumoniae infection dysregulate peripheral blood T lymphocyte subsets and ele-vate levels of TNF-α,IL-6,and IFN-γ.Moreover,peripheral blood lymphocyte subsets(CD4+,CD8+,CD4+/CD8+),and levels of TNF-α,IL-6,and IFN-γ can be used as an effective indicator for the clinical diagnosis of chil-dren with Mycoplasma pneumoniae infection,and the diagnostic accuracy of the combined data is better.
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