Value of targeted high-throughput sequencing in diagnosis of pathogens of lung infection
Objective To investigate the application value of targeted next-generation sequencing technology in the di-agnosis of pulmonary infection pathogens,and to provide a theoretical basis for clinical diagnosis.Methods A total of 124 patients with suspected pulmonary infection who were admitted to Zhengzhou Yihe Hospital affiliated to Henan Uni-versity from January 2023 to April 2024 were enrolled and sent for tNGS and traditional testing(microbial culture+PCR).The distribution of pathogenic microorganisms was counted,the pathogenic detection results of the two methods were compared,the positive rate and consistency of tNGS and traditional detection were analyzed,and the factors affect-ing the positive detection rate of tNGS were analyzed.Results Among the 124 patients,tNGS detected 50 species and 371 strains of pathogens,among which bacteria had the highest detection efficiency(45.01％),and tNGS also detected some special pathogens.tNGS can improve the typing of both resistance genes and viruses.The positive detection rate of tNGS(91.94％)was higher than the traditional method(62.90％),and the difference was statistically significant(P＜0.001).The detection rate of tNGS in bacterial,fungal,viral and co-infection was better than that of traditional detection,and the difference was statistically significant(P＜0.05).Conclusion Compared with the traditional test,tNGS has a higher detection rate of pathogens in the samples of patients with suspected lung infection,and can be effec-tively applied to the etiological diagnosis of lung infection.
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