Establishment and verification of serum adiponectin reference Interval in healthy people in Zhengzhou area
Objective To detect serum adiponectin(ADPN)levels in healthy people in Zhengzhou,and then analyze the gender and age differences and establish the reference interval for adiponectin of the population in this area.Methods According to the C28-A3 guideline of the American Clinical Laboratory Standardization Association(CLSI),the healthy physical examination population from the health management center of Henan Provincial Peoples Hospital from January 2023 to May 2023 were selected as the research subjects.After excluding people with metabolic diseases and abnormal lipid metabolism,a total of 948 compliantspecimens were collected.There were 430 male subjects and 518 female subjects,and the age was divided into five groups:18-30,331-40,41-50,51-60 and＞60 years old.Serum adiponectin levels were measured by latex enhanced immunoturbidimetry,and the reference interval of serum adiponectin was established by percentile method according to gender and age.Results Adiponectin levels showed a skewed distribution,The median value of serum adiponectin was 5.84 mg/L in male and 5.83 mg/L in female,the adiponectin levels did not differ between genders(Z=1.261,P=0.207);There were significant differences among the 5 age groups(H=32.060,P＜0.001),paired comparison showed that there was no significant difference among three age groups ≤50 years old and between the two age groups of＞50 years old(all P＞0.05).Therefore,And people over the age of 50 serum adiponectin levels are significantly higher than people under the age of 50(Z=4.757,P＜0.001).The range of adiponectin＞P25 was defined as the reference value.The overall adiponectin reference interval was＞4.71mg/L;≤50 years old adiponectin reference inter-val was 4.66 mg/L;＞50 years old adiponectin reference interval:＞5.03mg/L.The R value of 100 cases of healthy a-dults physical examination was 0.93,the newly established reference interval of serum adiponectin passed the verifica-tion.Multi-factor linear regression analysis showed that the serum adiponectin level was affected by age,fasting glu-cose,triglycerides,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol apolipoprotein E(stand-ardized regression coefficient beta=0.024,-0.693,2.193,-0.851,-0.399,0.297,P＜0.05).Conclusion This study established the reference interval of serum adiponectin in healthy people in Zhengzhou,which provided a reference standard for early screening of diabetes,metabolic syndrome and cardiovascular diseases.
Serum adiponectinReference intervalHealthy physical examination population
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