河南科技学院学报(自然科学版)2024,Vol.52Issue(1) :9-15.DOI:10.3969/j.issn.2096-9473.2024.01.002

低温处理对桃种子贮藏物质及抗氧化酶活性的影响

Effect of low temperature treatment on storage substances and antioxidant enzyme activity of peach seeds

侯小进 张梦 刘克帅 蔡祖国 陈莹莹 王保全
河南科技学院学报(自然科学版)2024,Vol.52Issue(1) :9-15.DOI:10.3969/j.issn.2096-9473.2024.01.002

低温处理对桃种子贮藏物质及抗氧化酶活性的影响

Effect of low temperature treatment on storage substances and antioxidant enzyme activity of peach seeds

侯小进 1张梦 1刘克帅 2蔡祖国 1陈莹莹 1王保全1
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作者信息

  • 1. 河南科技学院园艺园林学院,河南新乡 453003
  • 2. 新乡市果树研究所,河南新乡 453006
  • 折叠

摘要

桃种子打破休眠需要一定的需冷量,生产上常采用层积处理的方式打破桃种子的休眠.为了探究层积过程中物质含量的变化,以"锦香"和"秋彤"种子为试验材料,将破核后的桃种用湿毛巾包裹放入4 ℃条件下的冰箱,分别在15 d、30d、45 d、60 d取样,对其进行生理指标的测定.结果表明,"锦香"和"秋彤"种子的蛋白质含量在45 d时达到高峰,60 d时发生降低;可溶性糖含量随着低温处理时间的延长均呈现下降的趋势,表明可溶性糖含量与低温处理时间呈现显著的负相关;SOD酶活对照组均为最高,分别在30 d、45 d有上升的趋势;POD酶活与对照相比整体呈上升的趋势;MDA含量在45 d时均为最低.SOD、POD、MDA共同保护了细胞结构的完整性.通过对不同品种桃种子贮藏物质及抗氧化酶活性进行测定,分析贮藏物质和酶活性动态变化与桃种子休眠解除的关系.研究结果为明确桃种子萌发障碍以及休眠现象的产生提供重要的理论依据.

Abstract

Peach seeds need a certain amount of cold to break dormancy.In production,stratification treatment is usually used to break the dormancy of peach seeds.In order to investigate the changes of substance content in stratification process,seeds of"Jinxiang"and"Qiutong"were used as experimental materials.The peach seeds after breaking were wrapped with wet towels and placed in 4 ℃ refrigerator.Samples collected at 15 d,30 d,45 dand 60 d,were used to measure their physiological indicators.The results indicated that protein content of"Jinxiang"and"Qiutong"seeds peaked at 45 d and then decreased at 60 d.The soluble sugar content presented a decreasing trend with the extension of low temperature treatment time,indicating there is a significant negative correlation between soluble sugar content and low temperature treatment time.SOD enzyme activity was the highest in the control and increased at 30 d and 45 d,respectively;POD enzyme activity showed an increase compared to the control;MDA content was the lowest at 45 d.SOD,POD and MDA protected the integrity of the cell structure together.By measuring the storage substances and antioxidant enzyme activity of different varieties of peach seeds,the relationship between the dynamic changes of storage substances and enzyme activity and the release of peach seed dormancy had been analyzed.The results of the study will provide an important theoretical basis for identifying the barriers to germination and dormancy generation in peach seeds.

关键词

/种子休眠/低温处理/贮藏物质/抗氧化酶

Key words

peach/seed dormancy/low-temperature treatment/storage substances/antioxidant enzymes

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基金项目

国家重点研发计划项目(2018YFD1000300)

博士科研启动项目(207010618005)

出版年

2024
河南科技学院学报(自然科学版)
河南科技学院

河南科技学院学报(自然科学版)

影响因子:0.557
ISSN:1673-6060
参考文献量22
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