Study on the regeneration system of Gardenia jasminoides Ellis.var.radicans
Gardenia jasminoides Ellis.var.radicans is a fine landscape greening plant which has high research value and broad application prospect.Therefore,it is an important issue to establish the regeneration system of Gardenia jasminoides Ellis.var.radicans by plant tissue culture,which could set a system for the large-scale expanding propagation and production.Sterile leaves and terminal buds were selected as explants,the induction,differentiation of callus and rooting culture were carried out by adding different kinds and concentrations of plant growth regulators to screen out the best culture scheme.During initial culture of explant,the best combination of antibiotics to inhibit the contamination of bacteria was 50 mg/L ampicillin sodium+50 mg/L streptomycin sulfate(1:1)mixed solution with 25％pollution rate,better than that of the 200 mg/L cefotaxime solution and control group.During callus tissue induction,the first appeared time of callus for two kinds of explants(sterile leaves and sterile terminal buds)was 10 d and 18 d.The best combination plant growth regulators for callus tissue induction was MS medium+2.0 mg/L 2,4-D+0.5 mg/L 6-BA,and the induction rates were 90％(sterile leaves)and 93.3％(sterile terminal buds)respectively.During callus tissue differentiation,callus tissue differentiation rate was higher under moderate concentrations of NAA and higher concentration of 6-BA,and the best combination of plant growth regulators for callus differentiation was MS medium+0.05 mg/L NAA+1.8 mg/L 6-BA,and the differentiation rate reached 96.7％.During rooting-stage the best combination of plant growth regulators for rooting culture was 1/2 MS medium+0.1 mg/L NAA+0.5 mg/L IBA with 26.67％rooting rate.This study established a set of efficient regeneration system of Gardenia jasminoides Ellis.var.radicans,which would provide technical support for the large-scale expanding propagation,production and also create favorable conditions for its extensive popularization and application.
万方数据
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