In order to explore the optimal conditions for Sudan Ⅲ staining in the production of frozen sections of chicken fatty liver,this experiment took liver tissue from 7-day-old chickens and made them into sections using a frozen slicer.Then,different conditions were designed to stain the sections with Sudan Ⅲ staining solution,and the staining effect was observed and compared.The results showed that dyeing with Sudan Ⅲ dye for 9 min was significantly better than 5 and 30 min,washing with 70％alcohol 5 times was significantly better than 2 and 9 times,and the effect of blueing for 3 min was better than that of not blueing.Conclusion:The frozen section specimens of liver tissue were stained with Sudan Ⅲ for 9 min,washed 5 times with 70％alcohol,soaked with hematoxylin for 1 minute,and blued in tap water for 3 min.The fatty parts of liver tissue were significantly stained orange red,and the observation effect was the best.
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