Evaluation methods for lead tolerance of 37 maize varieties at seedling stage
In this study,37 maize varieties were taken as the research object,and seven traits of maize were analyzed,including plant height,SPAD value,fresh weight of plant,leaf length,leaf width,shoot dry weight,and root-shoot ratio.In order to establish an effective evaluation method for Pb tolerance of maize seedlings,the Pb tolerance coefficients of 7 traits of 37 maize varieties were converted into membership function values and D values by principal component and membership function analysis.Then,the Pb tolerance of maize seedlings was divided into five grades:high tolerance,tolerance,medium,sensitivity and high sensitivity through cluster analysis.Finally,the regression equation of D value and Pb tolerance coefficient of seven traits was established by regression analysis:D=-0.784+0.426*plant height+0.178*fresh weight of plant+0.126*shoot dry weight+0.361*SPAD+0.320*leaf length+0.041*leaf width-0.002*root-shoot ratio.The above results provide a basis for the evaluation of Pb tolerance at maizeseedling stage.