首页|紫甘蓝中花青素提取工艺及其抗氧化研究

紫甘蓝中花青素提取工艺及其抗氧化研究

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研究以紫甘蓝为原料,采用超声波辅助提取法优化花青素提取工艺,并分析花青素的抗氧化效果.通过单因素试验考察料液比、乙醇体积分数、超声时间对花青素提取量的影响,利用正交试验确定最佳提取工艺.结果表明提取花青素最佳的工艺条件为料液比为1∶25 g/mL、乙醇体积分数为55%、超声的持续时间为35 min,在此条件下紫甘蓝中花青素提取量为24.87 mg/g;体外抗氧化活性结果表明:同质量浓度下,提取物清除·OH能力>VC,提取物对DPPH·的清除率接近VC,且自由基清除能力与质量浓度成正比.
Extraction Technology and Antioxidant Research of Anthocyanins from Purple cabbage
Using purple cabbage as raw material,ultrasound was used to optimize the anthocyanin extraction process,and the antioxidant effect of anthocyanins was explored.The effects of solid-liquid ratio,ethanol volume fraction,and ultrasonic time on the extraction amount of anthocyanins were investigated through single-factor experiments,and the optimal extraction process was determined using orthogonal experiments.The results show that the optimal process conditions for extracting anthocyanins are:the solid-liquid ratio is 1∶25 g/mL,the ethanol volume fraction is 55%,and the duration of ultrasound is 35 minutes.Under these conditions,purple cabbage was measured.The mass concentration was 24.87 mg/g,and its antioxidant capacity was positively related to its concentration.The results of in vitro antioxidant activity showed that:at the same concentration,the ability of the extract to scavenge·OH>VC,the scavenging rate of the extract to DPPH was close to VC,and the free radical scavenging ability is proportional to the mass concentration.

PurplecabbageAnthocyaninacquisitionclearance rateoxidation resistance

张潇、周海旭、高晗、张玲玲

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河南科技学院食品学院,河南新乡 453003

漯河食品职业学院,河南漯河 462300

紫甘蓝 花青素 提取量 清除率 抗氧化能力

河南省科技攻关计划高层次人才科研项目(2017)

2021023101432017020

2024

河南科技学院学报(自然科学版)
河南科技学院

河南科技学院学报(自然科学版)

影响因子:0.557
ISSN:1673-6060
年,卷(期):2024.52(3)
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