Research progress on AtTUF gene of vacuolar H+-ATPase E1 subunit in Arabidopsis thaliana
Vacuolar type-H+-ATPase(V-ATPase,VHA)is a major type of proton pump present in all eukaryotes,mainly in the inner membrane systems of plant vacuoles,Golgi apparatus,and endosomes,which plays an important role in maintaining the pH homeostasis in the inner compartment of eukaryotes.V-ATPase is a protein complex composed of multi subunits,and it is devided into two major domains:the cytosolic-located hydrophilic V1 domain,which is comprising of eight different subunits(A～H),responsible for ATP hydrolysis,and a membrane-embedded hydrophobic V0 domain comprising of five subunits(a,c,c",d and e),is mainly responsible for proton tanslocation.In Arabidopsis thaliana,most subunits of V-ATPase are encoded by small gene families containing 2～5 members.Among them,VHA-E is encoded by three isotype genes with differential expression patterns.During the period of seed development,VHA-E1 is the major isotype during embryogenesis,which is necessary in the process of embryogenesis;VHA-E2 is pollen specific,and VHA-E3 mainly expresses in endosperm and the surrounding maternal tissues.The Arabidopsis V-ATPase subunit E1(VHA-E1)is encoded by AtTUF,which is closely related to the transport of substances across the vacuole membrane,plays an essential role in maintaining functional secretion system during the somatic development and in response to various abiotic stresses in plants.In this paper,the research progress of AtTUF gene in Arabidopsis thaliana was summarized,which may provide some reference for the research in related fields in the future.
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