Cloning and expression analysis of GmZAT10 gene in Soybean
Abiotic stress has a very serious impact on plant growth and development.Studies have found that there are a series of transcription factors in plants that can regulate abiotic stress,such as NAC,bZIP,WRKY,AP2/ERF,MYC,bHLH and C2H2.In order to reveal the function of GmZAT10 gene,gene cloning,vector construction,bioinformatics analysis,subcellular localization and real-time quantitative PCR(qRT-PCR)were carried out.The results showed that GmZAT10 was 702 bp in length and encoded 233 amino acids,The molecular weight is 59.14 kDa and the isoelectric point is 4.85.Subcellular localization showed that GmZAT10 was localized in the nucleus and cell membrane.GmZAT10 has two C2H2 zinc finger domains and contains QALGGH and C2H2 conserved sequences that are unique to plants.qRT-PCR was used to detect the expression of GmZAT10 gene in different soybean tissues and under different treatment conditions.The results showed that GmZAT10 gene was highly expressed in soybean roots and was induced by high and low temperature,NaCl and ABA.This study suggests that GmZAT10 play an important role in abiotic stress in soybean.
万方数据
维普
