In order to improve the conversion efficiency of corncob,the effects of different pretreatment conditions of two deep eutectic solvents,choline chloride/lactic acid and choline chloride/urea,on the enzymatic hydrolysis of corncob were investigated through orthogonal experiment.The results indicated that the maximum total reducing sugar concentration was 2.860 g/L when the pretreatment conditions of choline chloride/lactic acid were pretreatment temperature of 140 ℃,solid-liquid ratio of 1∶15,and pretreatment time of 1 h.The primary and secondary order of the effects of three factors on the enzymatic hydrolysis of corncob pretreated with choline chloride/lactic acid was pretreatment time,solid-liquid ratio,and pretreatment temperature.All three factors had no significant impact on enzymatic hydrolysis.The maximum total reducing sugar concentration was 2.941 g/L when the pretreatment conditions of choline chloride/urea were pretreatment temperature of 140 ℃,solid-liquid ratio of 1∶20,and pretreatment time of 2 h.The primary and secondary order of the effects of three factors on the enzymatic hydrolysis of corncob pretreated with choline chloride/urea was pretreatment temperature,pretreatment time,and solid-liquid ratio.The pretreatment temperature had a significant impact on enzymatic hydrolysis,while pretreatment time and solid-liquid ratio had no significant impact.The effect of different deep eutectic solvents and pretreatment conditions on the enzymatic hydrolysis of corncob varied.This study would provide a theoretical basis for the high-value utilization of lignocellulosic biomass.
维普
万方数据
