Effect of choline chloride deep eutectic solvent pretreatment on enzymatic hydrolysis of corncob
In order to improve the conversion efficiency of corncob,the effects of different pretreatment conditions of two deep eutectic solvents,choline chloride/lactic acid and choline chloride/urea,on the enzymatic hydrolysis of corncob were investigated through orthogonal experiment.The results indicated that the maximum total reducing sugar concentration was 2.860 g/L when the pretreatment conditions of choline chloride/lactic acid were pretreatment temperature of 140 ℃,solid-liquid ratio of 1∶15,and pretreatment time of 1 h.The primary and secondary order of the effects of three factors on the enzymatic hydrolysis of corncob pretreated with choline chloride/lactic acid was pretreatment time,solid-liquid ratio,and pretreatment temperature.All three factors had no significant impact on enzymatic hydrolysis.The maximum total reducing sugar concentration was 2.941 g/L when the pretreatment conditions of choline chloride/urea were pretreatment temperature of 140 ℃,solid-liquid ratio of 1∶20,and pretreatment time of 2 h.The primary and secondary order of the effects of three factors on the enzymatic hydrolysis of corncob pretreated with choline chloride/urea was pretreatment temperature,pretreatment time,and solid-liquid ratio.The pretreatment temperature had a significant impact on enzymatic hydrolysis,while pretreatment time and solid-liquid ratio had no significant impact.The effect of different deep eutectic solvents and pretreatment conditions on the enzymatic hydrolysis of corncob varied.This study would provide a theoretical basis for the high-value utilization of lignocellulosic biomass.
万方数据
维普
