Expression of BCAT1 in esophageal squamous cell carcinoma and its clinical signifi-cance
Objective To investigate the expression and clinical significance of branched-chain amino acid transam-inase 1(BCAT1)in esophageal squamous cell carcinoma(ESCC).Methods The qRT-PCR and Western blot were used to detect the expression level of BCAT1 gene in esophageal squamous cell lines,and suitable esophageal cancer cells were selected for follow-up study.The expression level of BCAT1 protein in ESCC cells was detected by Western blot after knock-down and overexpression of BCAT1.The proliferation,migration and invasion of ESCC cells were detected by CCK-8 activi-ty assay,clonogenesis assay,scratch assay and Transwell assay.Results The results of qRT-PCR and Western blot showed that:compared with normal cells,the expression of BCAT1 in ESCC was lower and significantly different,especially in KYSE150 and Eca109 cells.Therefore,these two cell lines were selected for study.The results of CCK-8 cell prolifera-tion experiment showed that the proliferation rate of CCK-8 cells after transfection with BCAT1 siRNA for 48 h was signifi-cantly higher than that of the control group.However,after overexpression of BCAT1,the effect was reversed.The results showed that knockdown of BCAT1 significantly promoted the cloning of ESCC cells,while overexpression of BCAT1 signifi-cantly inhibited the cloning of ESCC cells.The results of cell scratch test and Transwell test showed that the migration and invasion of ESCC cells in the BCAT1 siRNA group were significantly higher than those in the control siRNA group.The mi-gration and invasion of ESCC cells were significantly inhibited in the overexpression group.Conclusion The low expres-sion of BCAT1 in ESCC can significantly promote the proliferation,migration,invasion of ESCC cells.
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维普
