Expression of carbonic anhydrase Ⅸ in hepatocellular carcinoma and its relationship with clinicopathological characteristics
Objective To observe the expression of carbonic anhydrase Ⅸ(CAⅨ)in hepatocellular carcinoma(HCC)tissues,and to explore the clinical significance.Methods The differential expression levels of CAⅨ mRNA be-tween HCC tissues and paired liver tissues were analyzed by using GEPIA database,and the impact of CAⅨ expression on the prognosis of HCC patients were explored.From June 2021 to December 2023,45 HCC patients and 37 normal liver pa-tients who underwent liver puncture or surgery from the the First Affiliated Hospital of Henan University of Traditional Chi-nese Medicine were collected.The protein expression of CAⅨ was detected by immunohistochemistry method.The differen-tial protein expression of CAⅨ between HCC and normal liver tissues was analyzed.The correlation between the expression of CAⅨ and clinicopathological characteristics of the patients with HCC was discussed.Results The preliminary analysis results of the GEPTA database showed that the transcription level of CAⅨ in the HCC tissues was higher than that in the normal liver tissues(tumor tissues 369 cases,normal tissues 160 cases;t=8.532,P＜0.001),and the overall survival of HCC patients in the CAⅨ high expression group is significantly reduced compared to the CAⅨ low expression group(Log-rank P=0.002).Immunohistochemistry showed that the positive expression rate of CAⅨ in the HCC[55.56％(25/45)]was higher than that in the normal liver tissues[0.00％(0/37)],and the difference was statistically significant(x2=29.571,P＜0.001).The CAⅨ protein-expression had correlation with the histological grade of HCC,maximum tumor di-ameter,Ki-67 expression and phosphatidylinositol proteoglycan-3 expression(x2=4.549,P=0.033;x2=6.790,P=0.009;x2=4.664,P=0.031;x2=6.461,P=0.011).Conclusion The high expression of CAⅨ protein in HCC tissues may be related to the progression and poor prognosis of HCC,and is expected to become a potential diagnostic indicator and therapeutic target.
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