Construction of a prognostic prediction model for cervical squamous cell carcinoma of pyroptosis-related genes based on bioinformatics methods
Objective To investigate the role of pyroptosis-related genes(PRGs)in cervical squamous cell carci-noma(CSCC),to construct a prognostic model,and to analyze its correlation with immune infiltration of CSCC,so as to provide basis for prognosis prediction and personalized treatment of patients.Methods The RNA sequencing data and the corresponding clinical features from 253 CSCC patients were obtained from The Cancer Genome Atlas(TCGA)database.The RNA sequencing data from the normal human cervical samples were downloaded from the Genotype-Tissue Expression database.According to 26 PRGs identified differentially expressed between CSCC and normal cervical tissues,all the CSCC patients were divided into the high-risk group and the low-risk group.Through univariate,least absolute shrinkage and se-lection operator(LASSO),and multivariate Cox regression analyses,a three-gene signature was established.The validity of the signature was evaluated by decision curve analysis and Kaplan-Meier survival analysis.The underlying molecular mecha-nism of the PRGs in CSCC was discovered via gene enrichment analysis.the relationship between PRGs and immune cell in-filtration in CSCC patients were respectively analyzed.Results Three genes(GSDMB,IL1B,and PRKACA)among the 26 PRGs were identified to construct a prognostic signature[Risk score=(-0.215 7)× GSDMB+(0.241 8)× IL1B+(-0.221 9)× PRKACA)].The model divided CSCC patients into the high-risk group and the low-risk group,and the median risk score was taken as the cut-off value to evaluate the risk of CSCC,suggesting that the overall survival of CSCC patients with high-risk score was lower than that of CSCC patients with low-risk score(P=0.003).The area under curve of receiver operating characteristic curve of the prognostic model was 0.782,0.631 and 0.643,respectively,indicating that the prognostic model was a good predictor of the prognosis of CSCC.The establishment of a nomogram showed that the pre-dicted survival was in good agreement with the actual observation results.In addition,patients with high risk scores usually had lower levels of immune cell infiltration.Conclusion PRGs may play a critical role in CSCC and bring new insights to the underlying mechanisms of the cancer.
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