Effects of RNA interference on expression of receptor-interacting protein kinase 1 in clear cell renal cell carcinoma
Objective To explore the mechanism of RNA interference on the expression of receptor-interacting pro-tein kinase 1(RIPK1)gene in clear cell renal cell carcinoma.Methods After the expression of RIPK1 in ccRCC was re-duced by RNA interference technology,the morphological changes of cells were observed by light microscopy,the changes of cell proliferation were detected by CCK-8 method,the changes of cell migration ability were detected by Transwell cell migration assay,the level of apoptosis and necrosis was detected by flow cytometry,and the expression changes of cleaved caspase-3,mixed-lineage kinase-like domain(MLKL)and RIPK3 was detected by Western blot.Results The cell prolif-eration absorbance values of in the RIPK1-small interfering RNA(siRNA)group(0.563±0.042)was lower than that in the NC-siRNA group(0.944±0.039;t=11.550,P=0.003).The absorbance values of in the RIPK1-siRNA group(0.408±0.019)was lower than that in the NC-siRNA group(1.717±0.108;t=20.620,P<0.001).The cell migra-tion number of ccRCC in the RIPK1-siRNA group(31.660±10.020)was significantly lower than that in the NC-siRNA group(72.000±12.120;t=4.442,P=0.011).After siRNA interference with RIPK1,the expression levels of cleaved caspase3(0.780±0.070),MLKL(1.490±0.100),RIPK3(1.680±0.130)were significantly higher than those in the con-trol group(0.360±0.090,0.510±0.060,0.880±0.070;t=6.380,P=0.003;t=8.656,P=0.001;t=14.150,P=0.001).Conclusion Our findings highlighted that RIPK1 was indispensable for clear cell renal cell carcinoma.
clear cell renal cell carcinomareceptor-interacting protein kinase 1necropotosis
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